Porcine Aorta Isolation: A Surgical Procedure to Excise the Thoracic Aorta From Miniature Pigs for Downstream Analysis

Abstract

Source: Zhao, Y., et al. Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs J. Vis. Exp. (2019)

In this video, we demonstrate a procedure for the surgical removal of the aorta from miniature pigs. Endothelial cells harvested from these isolated aortae can be further cultured in in vitro conditions to study coagulation and immune responses.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgery and preparation for porcine aortic endothelial cell isolation

1. Disinfecting the operating room with UV light for 1 h and medical disinfectant 84 liquid (available chlorine content is 4.0% - 5.0%, Table of Materials) before performing surgery.
2. After the miniature pig received an intramuscular injection formed by 15 mg/kg Ketamine, 15 mg/kg Xylazine hydrochloride and 0.05 mg/kg Phenacetin hydrochloride (Volumes: 100μL/kg, Table of Material....

Results

Figure 1: The surgical procedure and excision of the aorta from a miniature pig. (A) Photograph of a miniature pig (Bama). (B) The aorta is exposed after laparotomy and thoracotomy. (C) The aorta is clamped.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
20 x PBS solution (pH 7.4, Nuclease free)	Sangon Biotech 	B540627#
75% Medical alcohol	Guilin LiFeng Medical Supplies Co., Ltd. China
Boneforceps	Beijing HeLi KeChuang Technology Development CO., Ltd. China	HL-YGQ
BOON Disposable Syringe (10ml)	Jiangsu Yile medical Article Co., Ltd. China
Compound ketamine injection	Veterinary Pharmaceutical Factory of Shenyang, China		Ketamine Hydrochloride: 0.3g/2ml, Xylazine hydrochloride:0.3g/2ml, Phenacetin hydrochloride: 1mg/2ml
Forceps 	ShangHai medical instruments Co., Ltd. China
Heparin sodium	Jiangsu WanBang biopharmaceutical Co., Ltd. China
Iodine tincture	Guilin LiFeng Medical Supplies Co., Ltd. China
Miniature Pig (Bama or Wuzhishan)	Kang Yi Ecological Agriculture Co., Ltd, China
Penicillin/Streptomycin 	Life Technologies 	15070063#
Scissors	ShangHai medical instruments Co., Ltd. China
Surgical scalpel	ShangHai medical instruments Co., Ltd. China	22#