Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

Abstract

Source: Hoover, J. L., et al., A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii. J. Vis. Exp. (2017)

This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Anesthetize, Intubate, and Inoculate Animals

NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 - 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with standard 12-hour light-dark cycles.

1. Prior to conducting any experiments, obtain and sterilize a metal cannula and polyethylene tubing of ....

Results

Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the hea.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Guide Tool	Intek Services Ltd	GT01
1 mL TB syringe slip tip	Becton Dickinson and Company	309659
PrecisionGlide Needle 25 G x 5/8	Becton Dickinson and Company	305122
Intramedic polyethylene tubing	Clay Adams brand - Becton Dickinson and Company	427401	Plastic cannula for mouse
Animal feeding needle - straight 20 x 3" 2-1/4	Popper and Sons, Inc	7903	Used to create metal cannula for mice
75 TN 5.0 µL syringe 26s/2"/2	Hamilton	87930	HPLC glass injection syringe
Difco Nutrient Agar	Becton Dickinson and Company	213000
Pyrex tube, culture 25x150 screwcap with rubber line	Corning	9825-25	To autoclave/store metal cannulae
PrecisionGlide Needle 25 G x 5/8	Becton Dickinson and Company	305122
70% isopropyl alcohol	Vi-Jon (Swan)	NDC 0869-0810-43
Isoflurane, USP	Piramal Healthcare	NDC 66794-013-10
50 mL Polypropylene Conical Tube 30 x 115 mm	Falcon, a Corning Brand	352070
75 TN 5.0 µL syringe 26s/2"/2	Hamilton	87930	HPLC glass injection syringe