A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E

Abstract

Source: Huang, Z., et al. Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE). J. Vis. Exp. (2019)

This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Basic information of the study group

NOTE: The Allergy Information Repository of the State Key Laboratory of Respiratory Disease (AIR-SKLRD) is a large serum bank established inside the Guangzhou Institute of Respiratory Hospital (GIRH). Initiated in the last decade, the AIR-SKLRD has already started collecting and storing serum samples from patients with allergic diseases and their clinical information (Table 1). The current study was performed with....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Agnitio BioIC Analyzer	Agnitio Science & Technology(Taiwan, China)	BA-G2000
BioIC Allergen specific-IgE Detection Kit	Agnitio Science & Technology(Taiwan, China)	DR17A12
BioIC Cartrideg Placement plate	Agnitio Science & Technology(Taiwan, China)	T20SET
BioIC Reagent Dispenser	Agnitio Science & Technology(Taiwan, China)	DS-1
Image two-dimensional barcode machine	Agnitio Science & Technology(Taiwan, China)	NLS-HR200
VORTEX-5 Vortex Mixer	Haimen Kylinbell Lab Lastruments Co., Ltd.	VORTEX-5