Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

Abstract

Source:
Rhea Kang¹, Gabby Mora¹, Sherry Dunbar<sup>1

1</sup>Luminex, A DiaSorin Company, Austin.

This video demonstrates multiplexed quantitative bacterial detection employing carboxylated magnetic polystyrene microspheres dyed into distinct sets. The procedure encompasses the formation of bead-bacteria complexes, magnetic separation, and flow-based analysis, enabling precise and simultaneous quantification of multiple bacteria in the same reaction well.

Protocol

1. Covalent Coupling of Capture Antibodies to MagPlex Microspheres

Each capture antibody is conjugated to a specific carboxylated magnetic microsphere set using a two-step procedure as described below.

NOTE: A coupling kit including all of the reagents, buffers, and materials required for microsphere coupling is also available (see TABLE OF MATERIALS). 

1. Resuspend the stock uncoupled microsphere suspension according to the instructions described in the product information sheet provided with your microspheres.
   NOTE: Resuspe....