Assessing Functional SARS-CoV-2 Neutralizing Antibodies

Abstract

Source:
Kelly Da Costa¹, Diego Cantoni^1,2, Martin Mayora-Neto¹, Tobia Fantoni^1,3, Nigel Temperton<sup>1

1</sup>Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent.

²MRC-University of Glasgow Centre for Virus Research, University of Glasgow.

³Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona.

This video showcases a neutralization assay measuring serum efficacy in neutralizing SARS-CoV-2 viruses. It involves pseudotyped viruses, serum dilution, and luminescence-based quantification, revealing the neutralization titer.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Pseudotype-based neutralization assay

NOTE: pMN is the Inhibition of pseudotype virus (PV) mediated transduction via human convalescent serum samples that contain antibodies directed against the SARS-CoV-2 Spike.

1. In a 96-well white plate with the aid of a multichannel pipette, add 50 µl of Dulbecco's Modified Eagle Medium (DMEM),10% fetaval bovine serum (FBS), 1% penicillin/streptomycin (Pen/Strep) to rows B to H, columns 1-12.
2. ....