An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor

Abstract

Source: Rwandamuriye, F. X. et al., A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing (Neo)adjuvant Therapies. J. Vis. Exp. (2020)

This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Inoculation of cells

1. Preparation of cells and animals
   1. Ensure that the cell line is maintained in the recommended media. For example, maintain WEHI 164 cell line in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum, 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 0.05 mM 2-mercaptoethanol, 100 U/mL penicillin, and 100 µg/mL streptomycin.
      NOTE: Passag....

Results

Figure 1: Representative images of the surgery setup. (A) A whole image of the surgery setup showing the surgical tools (listed in step 2.1) and the anesthetic machine. (B) A snapshot image of the surgical table showin.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
26 gauge 0.5 mL insulin syringe	Becton Dickinson, Australia	326769	None
2-Mercaptoethanol	Life Technologies Australia Pty Ltd	21985023	None
Anaestetic gas machine	Darvall Vet, Australia	SKU: 2848	None
Anti-CTLA-4	BioXcell, USA	BE0164	None
Anti-PD-1	BioXcell, USA	BP0273	None
Buprenorphine Hydrochloride Injection, 0.3mg/mL	RB healthcare UK Limited, UK	55175	Prescription order
Chlorhexidine Surgical Scrub 4%	Perigo Australia, Australia	CHL01449F(scrub	None
Fetal Bovine serum	CellSera, Australia	AU-FBS-PG	None
Forceps Fine 10.5 cm	Surgical house, Western Australia	CC74110	None
Forceps Fine 12 cm Serrated	Surgical house, Western Australia	CC74212	None
Forceps Halsted 14 cm	Surgical house, Western Australia	CD01114	None
Heating chamber	Datesand Ltd, UK	Mini-Thermacage	None
HEPES (1M)	Life Technologies Australia Pty Ltd	15630080	None
Isoflurane	Henry Schein Animal Health, Australia	SKU: 29405	Prescription order
Lubricating Eye Ointment	Alcon	n/a	None
Penicillin/streptomycin 1000X	Life Technologies Australia Pty Ltd	15140122	None
Phosphate Buffered Solution 10x	Life Technologies Australia Pty Ltd	70013-032	None
Reflex 7mm Clips	Able scientific, Australia	AS59038	None
Reflex 7mm Wound Clip Applicator	Able scientific, Australia	AS59036	None
Reflex Wound Clip Remover	Able scientific, Australia	AS59037	None
Rodent Qube Anesthesia Breathing Circuit	Darvall Vet, Australia	#7885	None
Roswell Park Memorial Institute (RPMI) 1640 Medium + L-glutamine	Life Technologies Australia Pty Ltd	21870092	None
Scissors Iris STR 11 cm	Surgical house, Western Australia	KF3211	None
Scissors Iris STR 9 cm	Surgical house, Western Australia	JH4209	None
Small Induction Chamber	Darvall Vet, Australia	SKU: 9630	None
TrypLE express 1x	Life Technologies Australia Pty Ltd	12604-021	None