A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs

In This Article

Abstract

Source: Phillips, J. E. et al., Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System. J. Vis. Exp. (2017)

This video demonstrates the generation of drug aerosols and the targeted nose-only delivery of these aerosols to mice using an inhalation unit equipped with an aerosol generator. The mouse is restrained in a nose-only restrainer and connected to the inhalation unit, which delivers the drug directly into the mouse's nasal cavity. Subsequently, the drug passes through the trachea and enters the lungs.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Formulation and Device Selection for the Generation of Pharmaceutical Aerosols

NOTE: Formulation and device selection depend on the individual drug's physicochemical properties to be aerosolized.

Nebulized liquid aerosols.
1. Dissolve the drug in 100 mL of water or physiologic saline.
2. Load a 100 mL syringe with the drug solution and place the syringe into the syringe pump with a flow ra....

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Results

Aerosol generator system diagram with flow path detail for inhalation study using animal model.
Figure 1: Inhalation unit with dry powder aerosol generator attached. The nebulizer used to generate aerosol from liquid formulations is shown on the right. 1) Wright dust feed, 2) cyclone, 3) syringe pump, 4) jet nebulizer inserted into pre-separator, 5) di.......