A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat

Abstract

Source: Greimers, L., et al. Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description. J. Vis. Exp. (2018).

This video demonstrates a technique for injecting platelet-rich plasma (PRP) into the sectioned Achilles tendon of rats. A small segment is excised from the Achilles tendon of a recipient rat, the site is sutured, and the rat is allowed to recover. Concurrently, whole blood is collected from a donor rat and centrifuged to obtain PRP, which is then activated and injected into the sutured site of the recipient rat to induce tendon repair.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Animal Preparation

1. Use 132 2-month-old male Sprague-Dawley rats weighing 320-450 g (120 rats for experimentation and 12 rats for blood sampling, Figure 1). Fifteen rats for each group was enough for a power of 0.8 (80% chance of finding statistical significance if the specified effect exists).
2. House all rats in classical cages under isolation conditions in a conventional facility equipped with a changing station. Instigate breeding in an IV....

Results

Figure 1: Experimental design of the study.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Xylazine (Xyl-M)	VMD	none	anesthetic
Ketamin (Jétamine 1000 CEVA)	CEVA Santé Animale	none	anesthetic
Buprenorphin (Vetergésic Multidosis)	ALSTOE	none	Painkiller
iso-Betadine	MEDA-Pharma	none	Desinfectant
Resorbable yarn Vicryl 6/0	Johnson & Johnson
Nembutal	CEVA Santé Animale	none	Anesthetic
Paraformaldehyde	Sigma-Aldrich	P6148	Preserves structure of the tissue
Isopropanol 100%	VWR	2,09,22,364
Ethanol 95%	VWR	2,08,23,362
Xylene	VWR	28973.363
Paraffin	VWR	LEIC3950.1006
Hematoxylin	Millipore	1.15938.0025	Colorant
Eosin	Millipore	1.15935.0100	Colorant
Eukitt	Sigma-Aldrich	3989	Mounting Medium
CaCl2