Generating a Traumatic Brain Injury Mouse Model Using an Impactor Probe

Abstract

Source: Furmanski, O., et al. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (2019).

This video demonstrates the procedure for inducing an open-skull traumatic brain injury in a mouse by making a midline skull incision, drilling through the skull and removing the bone flap to expose the brain cortex, and applying high pressure to induce trauma. The wound is then sutured to prepare the mouse model for subsequent experiments.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Craniectomy and controlled cortical impact

1. Preparation of the controlled cortical impact device and surgical supplies.
   1. Load a 1 mL slip-tip syringe with 0.5 mL of sterile saline for wound irrigation. Attach a 25 G needle to the syringe to control irrigation.
   2. Prepare a dilute solution of cyclosporine A (CsA) in dimethyl sulfoxide (DMSO) to a final concentration of 1 mg/mL. Load a second 1 mL slip-tip syringe with 0.5 mL of CsA solution for immun....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
1 ml syringes	Becton Dickinson (BD)	309659
25G Precision Glide syringe needles	Becton Dickinson (BD)	305122
anesthetic vaporizer	Vetland	0521-11-22
animal handling cloth			Purchase from department store
Betadine	Purdue Products	NDC-67618-151-32
compressed oxygen			Product of choice; varies by region
cyclosporine A	Sigma-Aldrich	30024-100mg
dimethyl sulfoxide (DMSO)	Sigma-Aldrich	D8418-500ml
DMEM	Invitrogen (ThermoFisher)	A14430-01
fine tweezers	Fine Science Tools	11254-20
forceps	Fine Science Tools	91106-12
High Speed Rotary Micromotor Kit	Foredom Electric Co.	K.1070 - K.107018
Ideal Micro Drill Burr Set Of 5	Cell Point Scientific	60-1000
Impact One Stereotaxic Impactor for CCI	Leica Biosystems	39463920
ophthalmic ointment	Falcon Pharmaceuticals	NDC-61314-631-36
small rodent stereotaxic frame	Stoelting	51925
small scissors	Fine Science Tools	14060-09
Sterile alcohol prep pads	Fisherbrand	06-669-62
sterile cotton swabs/Kendall Q-tips	Tyco Healthcare	540500
Sterile saline	Hospira	NDC-0409-1966-07
sutures - 5.0 silk with curved needle	Oasis	MV-682