Induction of Cerebral Microhemorrhages in a Rat Model Using Lipopolysaccharide

Abstract

Source: Li, D., et al. Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats. J. Vis. Exp. (2018).

This video demonstrates the process of inducing blood-brain barrier (BBB) disruption and cerebral microhemorrhages in a rat model using lipopolysaccharide (LPS). It highlights the sequence of immune responses that lead to increased BBB permeability and subsequent brain tissue damage.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Materials

1. Preparation of LPS injection​
   1. Add 25 mL of distilled water to 25 mg of LPS powder derived from Salmonella typhimurium to a final concentration of 1 mg/mL. Store the injection in a sterile tube at 4 °C.
      CAUTION: LPS is toxic.

2. Animals

1. Administer LPS to male Sprague-Dawley (SD) rats, aged 10 weeks (average w....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
LPS	Sigma-Aldrich	L-2630	for inflammation induction
Phosphate buffer saline (PBS)	Solarbio	P1022	a kind of buffer solution commonly used in experiment