Establishing a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury

Abstract

Source: Kim, M., et al. Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury. J. Vis. Exp. (2017).

The video demonstrates the procedure for creating a mouse model to study neonatal hypoxic-ischemic brain injury. It outlines the steps for surgically inducing ischemia and subjecting the animal to hypoxia to exacerbate brain damage. Finally, it shows how to assess the resulting brain lesions, ensuring the mouse model is prepared for further scientific investigation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.    

1. Mouse Model of Neonatal HI Brain Injury

1. Anesthetize the pups with isoflurane.
   1. Place the pups (less than 5) into an anesthetizing box and close the lid.
   2. Turn on the anesthetizing system for approximately 15 min; adjust the gas and isoflurane using a tabletop anesthesia machine. Adjust the oxygen flowmeter to 1.5 L/min. Adjust the isoflurane vaporizer to 3-5% for the induction of anesthesia.
   3. After 15 min, adjust the ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Hypoxic chamber	Jeung Do Bio & Plant Co	Experimental Builder
Harvard Apparatus Fluovac anesthetizing system	Harvard Apparatus
Anesthetizing box			acryl box
I-Fran Liquid (Isofluorane)	Hana Pharm. Co., Ltd.		General Anesthetics ( isoflurane 100ml)
CD-1 mice	Orient Co., Ltd.
Blue Nylon Mono Non-Absorbbable suture 5-0 50cm	Ailee Co., Ltd.	NB 521