Establishing a Focal Demyelination Mouse Model via a Two-Point Injection of Lysophosphatidylcholine

Abstract

Source: Pang, X., et al. A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice. J. Vis. Exp. (2022)

This video demonstrates the generation of a focal demyelination mouse model using a two-point Lysophosphatidylcholine (LPC) injection. The skull of an anesthetized mouse is exposed. The injection coordinates are determined, and a hole is drilled to enable LPC injections at targeted sites. LPC induces focal demyelination of neurons via inflammation, while a two-point injection ensures its sustainability.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Lysophosphatidylcholine (LPC) solution preparation

1. Dissolve 25 mg of LPC powder (see Table of Materials) with 250 µL of chloroform and methanol mixed solution (1:1) to make a 10% LPC solution and transfer it to a 500 µL centrifuge tube.
    NOTE: If the LPC is not completely dissolved, place the centrifuge tube in an ultrasonic cleaner and ultrasonicate at 40 kHz for ~1 h to obtain a uniform solution.
2. Div....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
L-α-Lysophosphatidylcholine from egg yolk	Sigma-Aldrich	L4129-25MG
32 gauge needle	HAMILTON	7762-05
10 μl syringe	HAMILTON	80014
High speed skull drill	strong,korea	strong204
Drill	Hager & Meisinger, Germany	REF 500 104 001 001 005
Matrx Animal Aneathesia Ventilator	MIDMARK	VMR
Portable Stereotaxic Instrument for Mouse	Reward	68507
Micro syringe	Reward	KDS LEGATO 130
Isoflurane	VETEASY
Suture	FUSUNPHARMA	20152021225
Adult C57BL/6 male and female mice	Hunan SJA Laboratory Animal Co. Ltd