Inducing Depression-like Behavior in a Mouse via Immobilization Stress

Abstract

Source: Son, H., et al., A Chronic Immobilization Stress Protocol for Inducing Depression-Like Behavior in Mice. J. Vis. Exp. (2019).

This video demonstrates the method of inducing chronic stress in a mouse through repeated immobilization, leading to corticosterone release and reduced glutamate receptor activity in neurons, which results in depression-like behavior.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Induction of depression by CIS restraint

NOTE: Handle the mouse gently but firmly with confidence. Rough and tentative handling is another stress factor in the experiment, and it is important for the mouse's struggling, biting, and scratching.

1. Set the room light to light (200 Lux) conditions using a digital lux meter.
2. House the mouse in a separate cage at least a week before testing and place the....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Ball nozzle	Jeung Do B&P	JD-C-88
CCTV camera	KOCOM	KCB-381
Digital lux meter	TES	TES-1330A
Mice	Koatech		C57BL/6 strain
Restrainer	Dae-jong Instrument Industry	DJ-428
Water bottle	Jeung Do B&P	JD-C-79