Experimental Glaucoma Induction via Iridocorneal Angle Obstruction In a Mouse Model

Abstract

Source: Bunker, S., et al. Experimental glaucoma induced by ocular injection of magnetic microspheres. J. Vis. Exp. (2015)

In this video, an anesthetized rat undergoes an ocular procedure where magnetic beads are injected into the anterior chamber under a magnetic field to block the iridocorneal angle. This blockage obstructs the trabecular meshwork, preventing aqueous humor drainage and leading to increased intraocular pressure, damaging the retinal ganglion cells and optic nerve. This develops a glaucoma-like condition.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.    

1. Ocular Hypertension Induction

1. Induce experimental glaucoma by elevating the intraocular pressure (IOP) via unilateral injection of paramagnetic microspheres into the anterior chamber of Brown Norway rats. Other pigmented rats may be suitable, although the user would need to validate them first.
2. House 250-300 g female ex-breeder Brown Norway rats in a constant low-light environment (40-60 lux) to minimize diurnal fluctuations in IOP, with access to fo....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
250-300g female Brown Norway ex-breeder rats	Harlan UK	203
Tonolab Rebound Tonometer	Tiolat	TV02
Ketaset (Ketamine)	Fort Dodge Animal health	BN1000118	37.5 mg/kg
Domitor (medetomidine hydrochloride)	Orion Pharma	140-999	0.25 mg/kg
Povidone iodine	Ecolab	BN4369LE10	5% in H2O
Minim's Saline Solution	Bausch and Lomb	PL00033/5017
Toroidal magnet	Supermagnete	R-10-07-03-N
Magnetic Microspheres	Bangs Laboratories	UMC4N/9692
HBSS	Invitrogen	14025
33-guage bevelled needle	Hamilton	7747-01	Custom needle
Luer tip syringe	Hamilton	80601
Antisedan (atipemezole hydrochloride )	Orion Pharma	141-003	0.25 mg/kg
Chloramphenicol ointment	Medicom	18956-0005