This video demonstrates a method to induce neuropathic pain in a rat model by constricting the infraorbital nerve. After exposing the infraorbital nerve and tying ligatures to constrict it, the immune response at the injury site increases the excitability of neighboring neurons, which transmit pain signals to the brain.
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Method Article
Induction of Neuropathic Pain in a Rat Model by Constriction of the Infraorbital Nerve
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April 28th, 2025
In This Article
Abstract
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Subjects
- Use male Sprague-Dawley rats (250-300 g at arrival).
- House rats in solid-bottom cages in a colony room with a humidity of 40-60% and a room temperature (RT) of 21 ± 1 °C.
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Chromic catgut | Dynek | CG504 | |
| Nembutal (pentobarbital) | Pharmacy | ||
| Atropine | Pharmacy | ||
| Clippers for small animals - Favorita II | Aesculap | GT104 | |
| Stereotaxic instrument | Stoelting | 51600 | |
| Ophthalmic ointment (Liposic) | Pharmacy | ||
| Homeothermic monitor system | Harvard Apparatus | PY8 50-7221-F | |
| Cotton applicator | Pharmacy | ||
| Precision cotton swab | Qosina | 10225 | |
| Operation microscope | Kaps | SOM 62 | |
| Dumont #5 forceps | Fine Science Tools | 11251-10 | |
| Dumont forceps - Micro-blunted tips (#5/45) | Fine Science Tools | 11253-25 | |
| Scissors - blunt tips | Fine Science Tools | 14574-09 | |
| Polyester sutures - Mersilene (4-0) | Ethicon | R871H |
