This video demonstrates the method for recording simultaneous plethysmography and electromyography signals in a freely moving ALS mouse model. The procedure involves using implanted electrodes and a transmitter to capture muscle activity, alongside a plethysmography chamber to monitor breathing patterns, to investigate the role of accessory muscles in the ALS mouse.
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Method Article
Simultaneous Plethysmography and Electromyography Recording in an ALS Mouse Model
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April 28th, 2025
In This Article
Abstract
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparing the Mouse for Surgery
- Choose the desired mouse for implantation (i.e., SOD1(G93A) or control) and weigh the animal.
NOTE: The recommended age and weight for a mouse (male or female) undergoing this surgery is P56 - P120 and....
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Results
Figure 1. Implantation of Telemetry Device to Measure Respiratory Muscle EMG.
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| B6.Cg-Tg (SOD1*G93A)1 Gur/J | Jackson Laboratory | 4435 | |
| Plethysmography Chamber | Buxco Respiratory Products/ Data Sciences International | 601-1425-001 | |
| Telemetry Receivers (Model RPC-1) | Data Sciences International | 272-6001-001 | |
| Bias Flow Pump (Model BFL0500) | Data Sciences International | 601-2201-001 | |
| ACQ-7000 USB | Data Sciences International | PNM-P3P-7002XS | |
| Dataquest A.R.T. Data Exchange Matrix | Data Sciences International | 271-0117-001 | |
| New Ponemah Analysis System | Data Sciences International | PNM-POST-CFG | |
| Ponemah Physiology Platform Acqusition software v5.20 | Data Sciences International | PNM-P3P-520 | |
| Ponemah Unrestrained Whole Breath Plethysmography analysis package v5.20 | Data Sciences International | PNM-URP100W | |
| Configured Ponemah Software System | Data Sciences International | PNM-P3P-CFG | |
| Analysis Module (URP) | Data Sciences International | PNM-URP100W | |
| Universal Amplifier | Data Sciences International | 13-7715-59 | |
| Sync Board | Data Sciences International | 271-0401-001 | |
| Sync Cable | Data Sciences International | 274-0030-001 | |
| Transducer-Pressure Buxco | Data Sciences International | 600-1114-001 | |
| Flow Meter | Data Sciences International | 600-1260-001 | |
| Magnet and Radio included in F20-EET Starter Kit | Data Sciences International | 276-0400-001 | |
| Axis P1363 Video Camera | Data Sciences International | 275-0201-001 | |
| Terg-A-Zyme | Fisher Scientific | 50-821-785 | Enzyme Detergent |
| Actril | Minntech Corporation | 78337-000 | Chemical Sterilant |
| Stereo Dissecting Microscope (Model MEB126) | Leica | 10-450-508 | |
| Servo-Controlled Humidifier/Infant Incubator | OHMEDA Ohio Care Plus | 6600-0506-803 | |
| TL11M2-F20-EET Transmitters | Data Sciences International | 270-0124-001 | |
| Dumont #2 Laminectomy Forceps - Standard Tips/Straight/12cm (x2) | Fine Scientific Instruments | 11223-20 | For handling wires |
| Dumont #2 Laminectomy Forceps - Standard Tips/Straight/12cm (x2) | Fine Scientific Instruments | 11223-20 | For surgery |
| Narrow Pattern Forceps- Serrated/Curved/12cm | Fine Scientific Instruments | 17003-12 | |
| Spring Scissors - Tough Cut/Straight/Sharp/12.5cm/6mm Cutting Edge | Fine Scientific Instruments | 15124-12 | |
| Tissue Separating Scissors - Straight/Blunt-Blunt/11.5cm | Fine Scientific Instruments | 14072-10 | |
| Fine Scissors - Tough Cut/Curved/Sharp-Sharp/9 cm | Fine Scientific Instruments | 14058-11 | For cutting wires and clipping nails |
| Scalpel Handle #3 | World Precision Instruments | 500236 | |
| Scalpel Blade | Fine Scientific Instruments | 10010-00 | For preparing lead caps |
| Polysorb Braided Absorbable suture | Coviden | D4G1532X | For coiling transmitter leads |
| Gluture | Zoetis Inc. | 6606-65-1 | Cyanoacrylate adhesive |
| 3 mL Syring Slip Tip - Soft | Vitality Medical | 118030055 | |
| 25G Needle (X2) | Becton Dickinson and Co. | 305-145 | |
| Cotton Tipped Applicators | Henry Schein Animal Health | 100-9175 | |
| Andis Easy Cut Hair Clipper Set | Andis | 049-06-0271 | Electrical Razor sold at Target |
| Isoflurane | Henry Schein Animal Health | 29404 | Anesthetic |
| Isopropyl Alcohol 70% | Priority Care 1 | MS070PC | |
| Dermachlor 2% Medical Scrub (chlorohexidine 2%) | Butler Schein | 55482 | |
| Artificial Tears | Henry Schein Animal Health | 48272 | Lubricant Opthalmic Ointment |
| Vacuum grease | Dow Corning Corporation | 1597418 | |
| Water Blanket | JorVet | JOR784BN |
