Developing Myopia in a Mouse Model Through Lens-Induced Defocusing

Abstract

Source: Jiang, X., et al. Inducement and evaluation of a murine model of experimental myopia. J. Vis. Exp. (2019)

This video demonstrates a procedure for inducing myopia in a mouse through the installation of a custom lens frame. The frame is secured to the exposed skull with dental adhesive, and concave lenses are inserted to shift the focal point behind the retina. This setup stimulates eyeball elongation leading to myopia.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Assembling the Eyeglasses for Mice

1. Prepare the parts needed for assembling the eyeglasses (Figure 1a). For each mouse, prepare all followings: one head-mounted nylon stick, one higher and one lower titanium frame, two lenses with proper powers according to the purpose of the experiment, one M1.4 size screw with a length of....

Results

Figure 1: The design of the skull-mounted eyeglasses. (a

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
screw	NBK	SNZS-M1.4-10
washer	MonotaRO	42166397
nut	MonotaRO	42214243
stick	DMM Make	none	designed by authers and output by the 3D printer rented from DMM Make.
frame	DMM Make	none	designed by authers and output by the 3D printer rented from DMM Make.
lenses	RAINBOW CONTACT LENS	none	customized for mice use by the company
cyanoacrylate glue	OK MODEL	MP 20g
dental adhesive resin cement	SUN MEDICAL	super bond	contains the etching liquid used for removing the periosteum of the mouse skull
Tropicamide, Penylephrine Hydrochloride solution	Santen	Mydrin-P
midazolam	Sandoz K.K.	SANDOZ	components for the anesthetic
medetomidine	Orion Corporation	Domitor	components for the anesthetic
butorphanol tartrate	Meiji Seika Pharma	Vetorphale	components for the anesthetic
0.1 % purified sodium hyaluronate	Santen	Hyalein
atipamezole hydrochloride	Zenoaq	antisedan