Inducing a Spinal Cord Contusion in a Mouse Model Using a Spinal Cord Injury Coaxial Platform

Abstract

Source: Elzat, E. Y. Y., et al., Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique. J. Vis. Exp. (2022).

This video demonstrates the method for inducing a contusion injury at the T9 level spinal cord in a mouse model, using a spinal cord injury coaxial platform. A weight is dropped on the T9 level spinal cord that creates a contusion that immediately causes tissue discoloration and swelling.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Locating and laminectomy of the 9^th thoracic vertebra (T9)

NOTE: Female C57BL/6J mice 9-10 weeks old were purchased from Jinan Pengyue Experimental Animal Company (Jinan, China).

Results

Figure 1. An imaging graph of the T13 costovertebral vertebra locating method. (A) The 13^th rib and T13 are relatively constant anatomical structures. The T13 costoverte.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Ethanol	Fuyu Reagent	64-17-5
Fluorescent microscope	KEYENCE	BZ-X800
Mice	Jinan Pengyue Experimental AnimalCompany	C57BL/6J
Microsurgery apparatus	Shandong ULT Biotechnology Co., Ltd	All the surgey instruments are custom-made	Ophthalmic scissors, micro mosquito forceps, microsurgery forceps, micro scissors
RWD Laboratory inhalation anesthetic station	RWD Life Science Co., Ltd	R550
Small animal in vivo microCT imaging system	PerkinElmer	Quantum GX2
Spinal cord injury coaxial platform	Shandong ULT Biotechnology Co., Ltd	Custom-made (Feng's standard)	(https://shop43957633.m.youzan.com/wscgoods/detail/367x5ovgn69q18g?banner_id=f.81386274~goods.7~1~ b0yRFKOq&alg_id=0&slg=tagGood List-default%2COpBottom%2Cuuid %2CabTraceId&components_style_ layout=1&reft=1659409105184&sp m=g.930111970_f.81386274&alias =367x5ovgn69q18g&from_uuid=136 2cc46-ffe0-6886-2c65-01903dbacbb a&sf=qq_sm&is_share=1&shopAuto Enter=1&share_cmpt=native_ wechat&is_silence_auth=1)