This video demonstrates monitoring interneuromast cell recovery in zebrafish using confocal microscopy, tracking cell death and repair through time-lapse fluorescence imaging.
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Method Article
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May 29th, 2025
This video demonstrates monitoring interneuromast cell recovery in zebrafish using confocal microscopy, tracking cell death and repair through time-lapse fluorescence imaging.
1. Post-ablation imaging and time-lapse microscopy to study regeneration
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 12-well PTFE Printed Slides | |||
| 15 mM Tricaine stock solution | |||
| 1X E3 + 600 µM Tricaine | |||
| 1X E3 media | |||
| 60 X E3 media | |||
| Bx60 Compound microscope with mercury arc lamp fluorescence | |||
| LSM 700 confocal microscope equipped with 405 nm, 488 nm, and 555 or 561 nm lasers | |||
| FIJI/ImageJ Image processing software | |||
| Dissecting needle modified into hair knife | |||
| Microsoft Excel software | |||
| Glass bottom 35 mm dishes with no. 1.5 coverslip, 20 mm window | |||
| Immersol 518F Immersion Oil | |||
| Low Gelling Agarose | |||
| Corning Netwell Insert with 74 um Polyester Mesh, 24 mm Insert | |||
| Rstudio software | |||
| SMX-168-BL Stereo microscope | |||
| Transfer Pipette | |||
| ZEN software |