In Vivo Imaging of the Mouse Retina Using Optical Coherence Tomography

Abstract

Source: Jagodzinska, J., et. al., Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo. J. Vis. Exp. (2017)

In this video, we demonstrate the use of spectral-domain optical coherence tomography (SD-OCT) to image retinal layers and assess structural changes in a genetically modified mouse model with optic neuropathy.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

Results

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Mice
Opa1delTTAG mouse	Institute for Neurosciences in Montpellier, INSERM UMR 1051, France	-	Opa1 knock-in mice carrying OPA1 c.2708_2711delTTAG mutation on C57Bl6/J background
Equipment
EnVisu R2200 SD-OCT Imaging System	Bioptigen, Leica Microsystems, Germany	-	Spectral-Domain Optic Coherence Tomography system
EnVisu R2200 SD-OCT Imaging System Software	Bioptigen, Leica Microsystems, Germany	-	Software for OCT acquisition and analysis
ImageJ 1.48v	Wayne Rasband, National Institutes of Health, USA	-	Software for analysis, requires downloading and installing two hommade macros: http://dev.mri.cnrs.fr/projects/imagej-macros/wiki/Retina_Tool
Self-regulating heating plate	Bioseb, France	BIO-062	Protection against hypothermia
Supplies
Gauze pads 3"x3"	Curad, USA	CUR20434ERB	Protection against hypothermia
Dual Ended Cotton tip applicator	Essence of Beauty, CVS Health Corporation, USA	-	Gel application
Cotton Twists	CentraVet, France	T.7979C.CS	Mouse positioning
Reagents and Drugs
Néosynéphrine Faure 10%	Laboratoires Europhtha, Monaco	-	Eye dilatation
Mydriaticum 0.5%	Laboratoires Théa, France	3397908	Eye dilatation
Cebesine 0.4%	Laboratoire Chauvin, Bausch&Lomb, France	3192342	Local anesthesia
Imalgene 1000	Merial, France/CentraVet, France	IMA004	General anesthesia
Rompun	Bayer Healthcare, Germany/CentraVet, France	ROM001	General anesthesia, analgesia, muscle relaxation
NaCl 0,9%	Laboratoire Osalia, France	103697114	Physiological serum
Systene Ultra	Alcon, Novartis, USA	-	Hydration of eyes
GenTeal'	Alcon, Novartis, USA	-	Ophtalmic gel to minimize light refraction and opacities
Aniospray Surf 29	Laboratoires Anios, France	59844	Disinfectant