Bioluminescence Imaging in an EAE Mouse Model

Abstract

Source: Schmitz, K., et al., Bioluminescence and Near-infrared Imaging of Optic Neuritis and Brain Inflammation in the EAE Model of Multiple Sclerosis in Mice. J. Vis. Exp. (2017).

This video demonstrates the method for visualizing and monitoring Experimental Autoimmune Encephalomyelitis or EAE-induced inflammation in the optic nerve using bioluminescence imaging. It includes positioning the mouse, reagent injection, and capturing sequential images to monitor changes in bioluminescence over time.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
XenoLight RediJect Inflammation Probe	Perkin Elmer, Inc., Waltham, USA	760535	Imaging probe, activatable by oxidases, useful for imaging of inflammation
PLP139-151/CFA emulsion	Hooke Labs, St Lawrence, MA	EK-0123	EAE induction kit
IVIS Lumina Spectrum	Perkin Elmer, Inc., Waltham, USA		Bioluminescence and Infrared Imaging System
LivingImage 4.5 software	Perkin Elmer, Inc., Waltham, USA	CLS136334	IVIS analysis software
Isoflurane	Abbott Labs, Illinois, USA	26675-46-7	Anaesthetic