This video demonstrates the use of fluorescence lifetime imaging microscopy (FLIM) to assess polyQ protein aggregation in C. elegans neurons, comparing control and chaperone protein-deficient worms. Increased aggregation in chaperone-deficient worms promotes energy transfer between clustered fluorophores, reducing fluorescence lifetime. FLIM-generated color-coded maps depict these differences, highlighting aggregation levels between experimental groups.
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Method Article
Fluorescence Lifetime Imaging of PolyQ Protein Aggregation in Caenorhabditis elegans Neurons
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June 17th, 2025
In This Article
Abstract
Protocol
1. Acquisition of FLIM data
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Results
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Ampicillin | |||
| B&H DCS-120 SPC-150 | |||
| B&H SPC830-SPC Image | |||
| C. elegans nQ40-CFP | |||
| Deckgläser-18x18mm | |||
| Leica M165 FC | |||
| Leica TCS SP5 | |||
| Levamisole Hydrochloride | |||
| PicoQuant PicoHarp300 | |||
| Sodium Azide | |||
| Standard-Objektträger | |||
| Universal Agarose | |||
| Zeiss AxioObserver.Z1 | |||
| Zeiss LSM510-Meta NLO |
