In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
Pressure myograph systems are exquisitely useful in the functional assessment of small arteries, pressurized to a suitable transmural pressure. The near physiological condition achieved in pressure myography permits in-depth characterization of intrinsic responses to pharmacological and physiological stimuli, which can be extrapolated to the in vivo behavior of the vascular bed. Pressure myograph has several advantages over conventional wire myographs. For example, smaller resistance vessels can be studied at tightly controlled and physiologically relevant intraluminal pressures. Here, we study the ability of 3rd order mesenteric arteries (3-4 mm long), preconstricted with phenylephrine, to vaso-relax in response to acetylcholine. Mesenteric arteries are mounted on two cannulas connected to a pressurized and sealed system that is maintained at constant pressure of 60 mmHg. The lumen and outer diameter of the vessel are continuously recorded using a video camera, allowing real time quantification of the vasoconstriction and vasorelaxation in response to phenylephrine and acetylcholine, respectively.
To demonstrate the applicability of pressure myography to study the etiology of cardiovascular disease, we assessed endothelium-dependent vascular function in a murine model of systemic hypertension. Mice deficient in the α1 subunit of soluble guanylate cyclase (sGCα1-/-) are hypertensive when on a 129S6 (S6) background (sGCα1-/-S6) but not when on a C57BL/6 (B6) background (sGCα1-/-B6). Using pressure myography, we demonstrate that sGCα1-deficiency results in impaired endothelium-dependent vasorelaxation. The vascular dysfunction is more pronounced in sGCα1-/-S6 than in sGCα1-/-B6 mice, likely contributing to the higher blood pressure in sGCα1-/-S6 than in sGCα1-/-B6 mice.
Pressure myography is a relatively simple, but sensitive and mechanistically useful technique that can be used to assess the effect of various stimuli on vascular contraction and relaxation, thereby augmenting our insight into the mechanisms underlying cardiovascular disease.
Pressure myograph systems are used to measure the physiological function and properties of small arteries, veins and other vessels. An intact small segment of an artery or vein is mounted onto two small glass cannulas and pressurized to a suitable luminal pressure, allowing the vessel to maintain most of its in vivo characteristics (Figures 1 and 2). The near physiological condition in a pressure myograph reflects the in vivo behavior of the vascular bed, allowing the investigation of intrinsic properties (e.g. myogenic tone) of isolated vessels. Some of the advantages of pressure myography over wire myography, where muscle contraction is assessed by direct mechanical coupling to a force transducer 1, include (i) that micro resistance arteries, which define the overall resistance developed in vascular system, can be studied, whereas wire myograph is limited to larger conduit arteries, (ii) that the risk to damage the endothelium is reduced since no wires need to be passed through the vessel lumen, (iii) that the natural shape of the vessel is better maintained, and (iv) that vessel dimension can be studied at a wide range of pressures or shear stress 2.
Studying micro vessels can be more informative than studying larger conduit arteries to help understand the pathophysiology and molecular mechanisms that contribute to altered vascular tone in cardiovascular diseases such as hypertension. For example, impairment of endothelial function associated with feeding mice a high fat diet for 8 weeks could be demonstrated in 2nd-order mesenteric arteries 3 but not in aortic rings (Figure 3). Another advantage of pressure myography is that intrinsic myogenic constriction of the pressurized vessel is present, and that the role and function of the endothelium in this phenomenon can be studied. Here, we describe the use of a pressure myograph to study vascular reactivity of mouse mesenteric 3rd order resistance arteries in a setting of impaired nitric oxide (NO)-cGMP signaling.
1. Preparation of Solution
2. Drugs Used
3. Mice
Male WT and sGCα1-/- mice on the S6 and B6 background were studied throughout this study. The sGCα1-/- were generated as described previously 4,5. Housing and procedures involving experimental animals (mice) were approved by the Subcommittee on Research Animal Care of Massachusetts General Hospital, Boston, MA.
4. Measurement of Vascular Reactivity in Mesenteric Artery
5. Statistical Analysis
All continuous measurements are expressed as mean ±SEM. The vascular reactivity in mesenteric arteries is analyzed by repeated-measures two-way ANOVA. In all cases, P < 0.05 is considered statistically significant.
NO is centrally involved in maintenance of blood pressure homeostasis both in humans 6 and in animal models 7. The ability of NO to control vascular smooth muscle relaxation is mediated by soluble guanylate cyclase (sGC), a heme-containing heterodimeric enzyme that generates cGMP 8. Recently, a blood pressure-modifying genetic variant was identified in a locus that contains the sGCα1 and sGCβ1 genes, illustrating the relevance of sGC in regulating blood pressure in humans 9. Interestingly, male mice deficient in the α1 subunit of sGC (sGCα1-/-) are hypertensive when on a 129S6 (S6) background (sGCα1-/-S6) 5 but not when on a C57BL/6 (B6) background (sGCα1-/-B6) 4. We used pressure myography to study vascular relaxation in response to acetylcholine in mesenteric resistance arteries isolated from WT and sGCα1-/- mice on the S6 and B6 backgrounds and preconstricted with phenylephrine (Figure 4). We studied resistance arteries because they define ultimate resistance and compliance of the vascular system and thus directly regulate blood pressure. sGCα1-deficiency was associated with a decreased ability of a cetylcholine to induce vascular relaxation, regardless of the genetic background of the mice studied (Figure 4 and 5). However, endothelium-dependent relaxation was more markedly impaired in sGCα1-/- mice on the S6 background than in sGCα1-/- mice on the B6 background (Figure 5) 10. Together, these findings suggest that the decreased sensitivity of the vasculature to endothelial-dependent relaxation may contribute to the strain-specific hypertension in sGCα1-/- mice.
Figure 1. Schematic depicting the various parts of the pressure myograph chamber (DMT). Inset shows a mounted vessels on cannulas in the chamber. Click here to view larger figure.
Figure 2. Representative images, captured during video recording, of a 2nd order mouse mesenteric artery under (A) basal and (B) phenylephrine-induced constriction. The outer diameter and vessel lumen are delineated by green and red lines, respectively. The traces in the right panels represent the signal intensity, measured in the area defined by the blue box in the images on the left, for outer diameter (green arrows) and lumen diameter (red arrows).
Figure 3. Endothelial dysfunction in mice fed with high-fat diet for 8 weeks was not apparent in aortic rings. Endothelial function was measured by the vasorelaxation response induced by acetylcholine ( 10-9 to 10-5 M) in phenylephrine ( 10-5 M) pre-contracted aortic rings. Standard diet, wild-type mice fed a standard diet (n=8); High-fat diet, wild-type mice fed a high-fat diet for 4-6 weeks (n=13).
Figure 4. Representative original tracings of a dose response curve of acetylcholine on phenylephrine-proconstricted 2nd order mesenteric arteries isolated from a WTB6 (A) and a sGCα1-/-S6 mouse (B). Arrows represent cumulative addition of increasing doses of acetycholine ( 10-9 to 10-5 M). The X axis represents time (in seconds), the Y axis represents lumen diameter (in μM). Click here to view larger figure.
Figure 5. The strain-specific hypertension in sGCα1-/- mice is associated with greater impairment of vascular reactivity in sGCα1-/-S6 mice than in sGCα1-/-B6 mice. The ability of acetylcholine ( 10-9 to 10-5) to induce relaxation is quantitated in phenylephrine-precontracted ( 10-5 M) mesenteric arteries from male wild-type (WT, closed symbols, full line) and sGCα1-/- (open symbnols, dashed line) mice on the B6 (circles) or S6 (squares) genetic background. Acetycholine-induced vascular relaxation is impaired to a greater extent in sGCα1-/-S6 than in sGCα1-/-B6 mice. N=3,5,3 and 6 for sGCα1-/-S6, sGCα1-/-B6, WTS6, respectively. †*P < 0.05 vs sGCα1-/-B6, P < 0.05 vs. sGCα1-/- of the same genetic background. Adapted form Buys, et al. (2012).
Mice are the experimental model of choice for many investigators, in part because of the possibility to introduce genetic modifications, thereby generating mouse models for human pathophysiology. The vasoactive status of small resistance but not of larger conduit vessels largely defines the regulation of blood flow throughout the vascular system 11. The size of resistance arteries in small animals, such as mice, prevents the use of a wire myograph to study microvascular function. Pressure myographs not only overcome this limitation but also allow for the measurement of vascular function under near physiological conditions.
We previously reported that male mice deficient in the sGCα1 are hypertensive when on a 129S6 (S6) background (sGCα1-/-S6) 5 but not when on a C57BL/6 (B6) background (sGCα1-/-B6) 4. We hypothesized that this strain-related disparity in blood pressure is due to the differences in vascular reactivity between sGCα1-/- mice on the B6 and S6 backgrounds. Using a pressure myograph, we studied endothelium-dependent vasorelaxation responses in 3rd order mesenteric arteries. Acetylcholine is a muscarinic receptor agonist that binds to its receptor in the endothelium to activate endothelial nitric oxide synthase (eNOS) via increasing intracellular Ca2+ concentration. NO can subsequently activate sGC in the underlying smooth muscle cells layer to induce relaxation.
Using pressure myography to compare the ability of endothelial derived NO to vasorelax mesenteric arteries isolated from WT and sGCα1-/- mice on the S6 and B6 background, we identified differences in vascular reactivity between sGCα1-/- mice on the B6 and S6 backgrounds 10. sGCα1-deficiency was associated with impaired endothelium-dependent vasorelaxation in both S6 and B6 mice. However, this impairment was greater in sGCα1-/-S6 than in sGCα1-/-B6 mice. The decreased ability of acetylcholine to induce vasorelaxation in S6 mice likely contributes to the increase in blood pressure observed in sGCα1-/-S6 mice but not in sGCα1-/-B6 mice. Although elevated blood pressure is often attributed to renal abnormalities 12, these results strengthen the emerging hypothesis that primary abnormalities of vascular smooth muscle tone, for example as associated with impaired NO-cGMP signaling 10,13, can directly contribute to the development of systemic hypertension 14.
In conclusion, the pressure myograph technique is a very powerful tool in the hands of the vascular physiologist or pharmacologist and can be used to analyze micro vessel function. Our study demonstrates the applicability of pressure myography in detecting underlying vascular abnormalities in a model of systemic hypertension.
The authors have nothing to disclose.
Authors would like to acknowledge Drs. Paul Huang and Dmitriy Atochin for use of DMT pressure myograph and Drs. Binglan Yu and Chong Lei for providing mice fed with high fat diet or standard diet.
Sources of Funding
This work was supported by Scientist Development Grant 10SDG2610313 from the American Heart Association (to E. S. Buys), and an Eleanor and Miles Shore 50th Anniversary Fellowship program for Scholars in Medicine from Harvard Medical School (to E. S. Buys).
Name | Company | Cataloge No. |
NaCl | Fisher Scientific | BP358 |
CaCl2 (2H2O) | Fisher Scientific | C79-500 |
KCl | Sigma | P9333 |
MgSO4 | Fisher Scientific | M65-500 |
KH2PO4 | Sigma | P3786 |
NaHCO3 | Fisher Scientific | BP328 |
NaOH | Fisher Scientific | S318 |
D-Glucose | Sigma | G8270 |
EDTA | Fisher Scientific | BP121 |
HEPES | Sigma | H3375 |
Phenylephrine | Acros Organics | AC20724 |
Acetylcholine | Sigma | A6625 |
Pressure Myograph System | DMT |