Accuracy is a major interest in many fields in dental medicine. Replacing dental hard tissue needs an exact fitting prosthesis to ensure proper function and prevent further destroying the remaining tooth structure1,2. Fixed partial dentures and total prosthesis are especially critical for exact fitting at supporting structures like prepared teeth or implants3. This is why a highly accurate reproduction is needed, especially in the field of dental impressions and dental laboratory workflow. However, other fields of dental treatment also benefit from a true and precise metrical outcome, to verify treatment success and evaluate new treatment strategies, e.g. soft and hard tissue augmentation, erosion and abrasion monitoring, periodontal treatments, and orthodontic treatments4,5. In many of these fields, current validation procedures are linear distance measurements with calipers or microscopes6,7. These methods are limited to only few measuring points and limited information of three-dimensional (3D) changes of the testing area. Newer measuring methods include the optical or radiographic capturing of the entire surface of the test object8,9. Here, the entire surface or volume is measured and displayed as a 3D object on the computer screen. Linear measurements are possible, as well as superimpositions of models from different scan times. With this superimposition, an evaluation of the surface changes at every scan point is possible. This enables the possibility of monitoring a specific area or displaying deformations in all three coordinate axes. Also, volumetric changes can be measured10. The limiting point with these new methods is the accuracy of the scanner, used to capture the test object. None of the changes within the accuracy of the reference scanner can be divided into changes of the test object or scan errors. Scan accuracy is often a value given by the manufacturer derived from scanning small, calibrated objects11. This minimal scan error is different when scanning large objects like a dental arch. Accuracy consists of trueness and precision. Trueness is the deviation of the scanned object from its real geometry. Precision is the deviation between repeated scans (ISO 5725-1). In this study, a new optical reference scanner, based on the focus variation scanning technique, was introduced to scan specimens from single tooth up to full arch models with highest accuracy. This reference scanner was used as a base for several studies, comparing dental impression accuracy from conventional and digital techniques12–14 and for actual projects concerning dental occlusion and abrasion of dental materials. The aim of this study was to provide basic information of the accuracy of the reference scanner and some possibilities to use this device in the field of dental research.