Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Summary

The goal of this protocol is to use temperature to control the flow speeds of three-dimensional active fluids. The advantage of this method not only allows for regulating flow speeds in situ but also enables dynamic control, such as periodically tuning flow speeds up and down.

Abstract

We present a method for using temperature to tune the flow speeds of kinesin-driven, microtubule-based three-dimensional (3D) active fluids. This method allows for tuning the speeds in situ without the need to manufacture new samples to reach different desired speeds. Moreover, this method enables the dynamic control of speed. Cycling the temperature leads the fluids to flow fast and slow, periodically. This controllability is based on the Arrhenius characteristic of the kinesin-microtubule reaction, demonstrating a controlled mean flow speed range of 4–8 µm/s. The presented method will open the door to the design of microfluidic devices where the flow rates in the channel are locally tunable without the need for a valve.

Introduction

Active matter is differentiated from conventional passive matter due to its capability to convert chemical energy into mechanical work. A material that possesses such capability can consist of living or non-living entities such as bacteria, insects, colloids, grains, and cytoskeletal filaments^{1,2,3,4,5,6,7,8,9,10}. These material entities interact with their neighbors. At a larger scale, they self-organize into either turbulent-like vortices (active turbulence) or material flows^{11,12,13,14,15,16,17,18,19,20}. An understanding of self-organization of active matter has led to various applications in molecular shuttles, optical devices, and parallel computation^21,22,23. To bring applications to the next level requires control beyond self-organization. For example, Palacci et al. developed a hematite-encapsulated colloid that self-propelled only when exposed to manually controlled blue light, which led to the emergence of living crystals²⁴. Morin et al. established the control of rolling Quincke colloids by using a tunable external electric field, resulting in colloidal flocking in a racetrack-like channel²⁵. These previous works demonstrate the role of local control in applications and advance the knowledge base of active matter.

In this article, we focus on the controllability of kinesin-driven, microtubule (MT)-based 3D active fluids. The fluids consist of three main components: MTs, kinesin molecular motors, and depletants. The depletants induce a depletion force to bundle the MTs, which are later bridged by motor clusters. These motors walk along the MTstoward the plus end. When a pair of bridged MTsis antiparallel, the corresponding motors walk in opposite directions. However, the motors are bound in a cluster and are unable to walk apart, so they cooperatively slide apart pairs of MTs (interfilament sliding, Figure 1A). These sliding dynamics accumulate, causing bundles of MTsto extend until reaching their buckling instability point and break (extensile bundles, Figure 1B)²⁶. The broken bundles are annealed by the depletion force, which subsequently extends again, and the dynamics repeat. During the process of the repeating dynamics, the bundle movements stir the nearby liquid, inducing flows that can be visualized by doping with micron-scale tracers (Figure 1C). Sanchez et al. and Henkin et al. have characterized the mean speeds of tracers, finding that the speeds were tunable by varying the concentrations of adenosine triphosphate (ATP), depletants, motor clusters, and MTs^19,27. However, such tunability existed only prior to active fluid synthesis. After synthesis, the tunability was lost, and the fluids self-organized in their own way. To control active fluid activity after synthesis, Ross.et al. reported a method using the light-activated dimerization of motor proteins, allowing fluid activity to be tuned on and off using light²⁸. While light control is convenient in terms of locally activating the fluids, the method requires redesigning the structures of motor proteins, along with modifying the optical paths in a microscope. Here, we provide an easy-to-use method for locally controlling fluid flows without microscope modification while keeping the motor structure intact.

Our method of locally tuning active fluid flow is based on the Arrhenius law because the kinesin-MT reaction has been reported to increase with temperature^29,30,31,32. Our previous studies showed that the temperature dependence of the mean speed of an active fluid flow followed the Arrhenius equation: v = A exp(-E_a/RT), where A is a pre-exponential factor, R is the gas constant, E_a is the activation energy, and T is the system temperature³³. Therefore, fluid activity is sensitive to the temperature environment, and the system temperature needs to be consistent to stabilize the motor performance, and consequently, the fluid flow speed³⁴. In this article, we demonstrate the use of the motor's temperature dependence to continuously tune the flow speeds of active fluids by adjusting the system temperature. We also demonstrate the preparation of an active fluid sample, followed by mounting the sample on a microscope stage whose temperature is controlled via computer software. Increasing the temperature from 16 °C to 36 °C speeds up the mean flow speeds from 4 to 8 µm/s. Additionally, the tunability is reversible: repeatedly increasing and decreasing the temperature sequentially accelerates and decelerates the flow. The demonstrated method is applicable to a wide range of systems where the main reactions obey the Arrhenius law, such as the MT gliding assay^29,30,31,32.

Protocol

1. Preparation of MTs

CAUTION: In this step we purify tubulins from bovine brain tissue. Bovine brain may cause variant Creutzfeldt-Jakob disease (vCJD)³⁵. Therefore, the brain waste and related solutions, bottles, and pipette tips should be collected in a biowaste bag and disposed of as biohazardous waste according to the rules of the institution.

1. Purify tubulins from bovine brain (modified from Castoldi et al.³⁶).
   1. Transport approximately 1.5 kg of fresh bovine brains from a local slaughterhouse to a university cold room. During transport, store the brains in phosphate buffer (20 mM NaH₂PO₄, 150 mM NaCl, pH 7.2) on ice.
      NOTE: To maximize the final yield of tubulin, the initial amount of functional tubulin in the fresh brain tissue is key. Fresher brains contain more functional tubulin. To obtain the freshest brains from the slaughterhouse, we recommend asking the butcher to provide brains from the most recently slaughtered cows. Brains should be no older than 3 h when starting the procedure, because reducing the time between slaughter and procedure start will produce better yields.
   2. Homogenize and clarify the brains.
      1. Clean the brain by using scalpels to cut blood vessels and connective tissue into smaller pieces and remove them from the brain by hand.
         NOTE: The cleaned brains should be pink.
      2. Immerse the cleaned brain tissue in 1 L of depolymerization buffer (DB: 50 mM 2-(N-morpholino) ethanesulfonic acid, 1 mM CaCl₂, pH 6.6) per kilogram of brain.
      3. Homogenize the brains with a kitchen blender.
      4. Centrifuge the homogenized brain solution at 10,000 x g and 4 °C for 150 min.
   3. Polymerize MTs (first polymerization).
      1. Collect and mix the supernatant with equal volumes of the following solutions at 37 °C: glycerol, high-molarity PIPES buffer (HMPB: 1 M PIPES, 10 mM MgCl₂, 20 mM ethylene glycol-bis(β-aminoehyl ether)-N,N,N',N'-tetraacetic acid [EGTA], pH 6.9)
      2. Add 1.5 mM ATP and 0.5 mM guanosine triphosphate (GTP) to the mixture.
      3. Incubate the mixture at 37 °C for 1 h.
   4. Depolymerize MTs (first MT depolymerization).
      1. Pellet MT by centrifuging at 151,000 x g and 37 °C for 30 min.
      2. Discard supernatant and resuspend each MT pellet in 10 mL of 4 °C DB.
      3. Incubate on ice for 30 min.
      4. Agitate the mixture every 5 min with a pipette tip to avoid MT sedimentation.
         NOTE: The mixture should turn clear in 30 min, indicating completion of MT depolymerization.
   5. Polymerize MTs (second MT polymerization).
      1. Clarify the solution by centrifuging at 70,000 x g and 4 °C for 30 min.
      2. Collect and mix the supernatant with equal volumes of 37 °C glycerol and HMPB.
      3. Add 1.5 mM ATP and 0.5 mM GTP to the mixture.
      4. Incubate the mixture at 37 °C for 30 min.
   6. Repeat step 1.1.4, replacing HMPB with Brinkley reassembly buffer (BRB) 80 (80 mM PIPES, 1 mM MgCl₂, 1 mM EGTA, pH 6.8), followed by clarifying the cleared mixture by centrifuging at 79,000 x g and 4 °C for 30 min.
   7. Collect the supernatant. Measure the 280 nm absorbance with a spectrometer. Determine the tubulin concentration using the Beer-Lambert law (extinction coefficient of tubulin: 1.15 (mg/mL)^-1cm^-1)^37,38.
   8. Store the protein at -80 °C.
2. Recycle tubulins (modified from Castoldi et al.³⁶).
   NOTE: To enhance tubulin purity, the purified tubulin is polymerized and depolymerized again.
   1. Polymerize the MTs by mixing the purified tubulin (step 1.1.7) with 500 µM dithiothreitol (DTT) and 20 µM GTP, followed by 30 min of incubation at 37 °C.
   2. Pellet the MTs.
      1. Load 1 mL of glycerol cushion (80 mM PIPES, 1 mM MgCl₂, 1 mM EGTA, 60% v/v glycerol, pH 6.8) in the bottom of a centrifuge tube.
      2. Lay the polymerized MTs on top of the cushion.
      3. Centrifuge at 172,000 x g for 90 min at 37 °C.
   3. Depolymerize the MTs.
      1. Remove the supernatant and cushion.
      2. Resuspend each MT pellet in 150 µL of 4 °C MT buffer (M2B: 80 mM PIPES, 2 mM MgCl₂, 1 mM EGTA, pH 6.8).
      3. Incubate the mixture on ice for 30 min.
      4. Agitate the mixture with a pipette tip every 5 min. The mixture should turn clear.
   4. Clarify the mixture by centrifuging at 172,000 x g and 4 °C for 30 min.
   5. Collect the supernatant. Measure the protein concentration. Store at -80 °C.
3. Label the tubulin with fluorescent dye³⁹.
   1. Polymerize the MTs. Mix the purified tubulin (step 1.1.7) with 500 µM DTT and 16.7 µM GTP, and incubate the mixture at 37 °C for 30 min.
   2. Pellet the MTs.
      1. Place 1 mL of 37 °C high-pH cushion (0.1 M NaHEPES, 1 mM MgCl₂, 1 mM EGTA, 60% v/v glycerol, pH 8.6) into a centrifuge tube.
      2. Lay the polymerized MTs on the cushion.
      3. Centrifuge at 327,000 x g for 50 min at 37 °C.
   3. Label tubulin.
      1. Resuspend each MT pellet with 700 µL of 37 °C labeling buffer (0.1 M NaHEPES, 1 mM MgCl₂, 1 mM EGTA, 40% v/v glycerol, pH 8.6).
      2. Mix the suspension with a 10–20 molar excess of far-red fluorescent dye functionalized with a succinimidyl ester.
      3. Incubate the mixture at 37 °C for 30 min in the dark to allow the MTs to react with the ester of the fluorescent dye.
      4. Stop the labeling reaction by saturating the ester of the suspending dye with 50 mM K-glutamate, incubating for 5 min at 37 °C.
   4. Pellet the labeled MTs: Repeat step 1.3.2, replacing the high-pH cushion with low-pH cushion (80 mM pipes, 2 mM MgCl₂, 1 mM EGTA, 60% v/v glycerol, pH 6.8).
   5. Depolymerize MTs.
      1. Discard the supernatant and the cushion.
      2. Resuspend the pellet in 700 µL of 4 °C M2B.
      3. Incubate the suspension on ice.
      4. Agitate every 5 min with a pipette tip until the solution is clear.
   6. Clarify the cleared solution by centrifuging at 184,000 x g and 4 °C for 35 min.
   7. Enhance the purity of the labeled tubulin solution by repeating steps 1.2.1–1.2.4.
   8. Measure the concentration of proteins and fluorescent dye. Use these measurements to determine the tubulin concentration and the fractions of labeled tubulin, defined as the ratio of concentrations of fluorescent dye to tubulin.
   9. Store the labeled tubulin solution at -80 °C.
4. Polymerize the MTs (adopted from Sanchez et al.¹⁹):
   1. Mix the recycled tubulin (step 1.2.5) with labeled tubulin (step 1.3.9) in a ratio that yields a 3% labeled tubulin fraction.
   2. Mix the 8 mg/mL tubulin mixture with 1 mM DTT and 0.6 mM guanosine-5'[(α,β)-methyleno]triphosphate (GMPCPP), followed by 30 min of incubation at 37 °C.
   3. After the incubation, anneal the MTs at room temperature in the dark for 6 h.
   4. Aliquot and store at -80 °C.

2. Synthesize kinesin clusters

NOTE: Bacteria exist ubiquitously and can grow in the media and contaminate the preparation process. To prevent contamination, actions involving contact with the cell cultures (e.g., pipetting) MUST be performed near a flame. Tools such as flasks, pipettes, pipette tips, media, and plates MUST be autoclaved before use.

1. Express kinesin motors in Escherichia coli⁴⁰.
   1. Transform the cells.
      1. Pipette 1 µL of the K401-BCCP-H6 plasmid into 10 µL of competent cells.
      2. Incubate on ice for 5 min.
      3. Heat-shock at 42.5 °C for 45 s.
      4. Incubate the cells on ice for 2 min to allow recovery.
      5. Mix the cells with 300 µL of antibiotic-free 2XYT (5 g/L NaCl, 10 g/L yeast extract, 16 g/L tryptone).
      6. Incubate the cells at 37 °C for 1 h.
         NOTE: Unless specified, monitoring the cell growth is not required during incubation.
   2. Inoculate plate media.
      1. Spread the cell culture on a 2XYT plate (10 g/L NaCl, 5 g/L yeast extract, 10 g/L tryptone, 15 g/L agar, 100 µg/mL ampicillin, 25 µg/mL chloramphenicol).
      2. Incubate the plate upside down at 37 °C overnight.
   3. Inoculate liquid media.
      1. From the overnight plate, harvest one isolated colony with a pipette tip.
      2. Eject the tip into a flask containing 50 mL of 2XYT.
      3. Incubate and shake the culture media at 37 °C and 200 rpm for 12–16 h.
   4. Expand the cells.
      1. Mix 2.5 mL of the cell culture in 500 mL of 2XYT.
      2. Incubate and shake the 500 mL culture at 37 °C and 250 rpm for 3–6 h.
   5. Induce protein expression.
      1. During the incubation, monitor cell growth by measuring the absorbance at 600 nm, using 2XYT as a reference. Measure the absorbance every 60 min, until it reaches OD₆₀₀ = 0.3. Then measure the absorbance every 30 min until it reaches 0.5–0.6.
      2. Allow the cells to grow until the absorbance reaches OD₆₀₀ = 0.5–0.6
      3. Add 24 µg/mL biotin and 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) to the cell culture.
         NOTE: IPTG is used to induce protein expression of the kinesin motors, which are tagged with biotin carboxyl carrier protein (BCCP) and six histidines (H6). The H6 tag is used in the purification process (step 2.2), while BCCP binds to the added biotin molecules to biotinylate the expressed kinesin motors.
      4. Incubate and shake the cell culture at 20 °C and 250 rpm for 12–20 h.
   6. Harvest the cells by centrifuging at 5,000 x g and 4 °C for 10 min. Discard the supernatant. Store the cell pellets at -80 °C.
2. Purify the kinesin motor protein (modified from Spriestersbach et al.⁴¹):
   1. Suspend the cell pellet (step 2.1.6) with an equal volume of lysis buffer (50 mM PIPES, 4 mM MgCl₂, 50 µM ATP, 10 mM 2-mercaptoethanol (βME), 20 mM imidazole, pH 7.2), followed by adding one tablet of protease inhibitor, 2 mg of phenylmethyl sulfonyl fluoride (PMSF), and 2 mg of lysozyme.
   2. Lyse the cells by flash freezing in liquid nitrogen (LN) 3x and thawing the cell mixture.
      NOTE: After lysing, the mixture should become viscous.
   3. Clarify the lysed cell mixture by centrifuging at 230,000 x g and 4 °C for 30 min.
   4. Collect the supernatant and flow it through a gravity column, followed by washing the column with 10 mL of lysis buffer.
      NOTE: Proteins with an H6 tag, such as kinesin K401-BCCP-H6, should remain in the column.
   5. Eluate the tagged protein with 5 mL of elution buffer (50 mM PIPES, 4 mM MgCl₂, 50 µM ATP, 500 mM imidazole, pH 7.2). Collect the flow-through sequentially in 1 mL fractions. To determine the protein-containing fractions, mix 3 µL of each fraction with 100 µL of triphenylmethane dye. The protein-containing fractions should turn blue. Combine these fractions and dilute by 5x with lysis buffer.
   6. Concentrate the protein solution.
      1. Load the solution in a centrifugal filter tube.
      2. Centrifuge at 3,000 x g for 10 min at 4 °C.
      3. Shake the tube gently and centrifuge again until the solution volume is <3 mL.
   7. Measure the protein concentration with a spectrometer (extinction coefficient: 0.549 (mg/mL)^-1cm^-1)^42,43. Dilute the protein to 1 mg/mL while adding 35% w/v sucrose.
   8. Store at -80 °C.
3. Measure the kinesin concentrations with an electrophoresis gel (modified from Taylor et al.⁴⁴).
   NOTE: To measure the kinesin concentration with an electrophoresis gel, tubulin is an ideal concentration ladder because of its high purity and its measurable concentration via a spectrometer (Figure 2A)³⁶.
   1. Prepare tubulin samples with concentrations of 0.25, 0.5, 0.75, 1.00, and 1.25 mg/mL. Mix 15 µL of each tubulin sample and kinesin sample (step 2.2.8) separately with 5 µL of sample buffer (200 mM Tris-HCl, 8% sodium dodecyl sulfate (SDS), 400 mM DTT, 0.2% bromophenol blue, 40% glycerol, pH 6.8) and incubate at 90 °C for 3 min.
   2. Prepare the electrophoresis.
      1. Lock an electrophoresis gel into the gel box.
      2. Fill the box with running buffer (50 mM MOPS, 50 mM Tris base, 0.1% SDS, 1 mM ethylenediaminetetraacetic acid (EDTA), pH 7.7).
         NOTE: Ensure that the buffer level is above the top opening of the gel.
   3. Load 10 µL of protein standard ladder, tubulin samples, and kinesin sample into separate wells and apply 200 V across the gel for 45 min.
   4. Gel staining.
      1. Incubate and rock the gel with boiled (approximately 95 °C) stain solution A (0.5 g/L triphenylmethane dye, 10% v/v acetic acid, 25% isopropanol) for 5 min, then rinse the gel with deionized (DI) water.
      2. Repeat with stain solutions B (0.05 g/L triphenylmethane dye, 10% v/v acetic acid, 10% isopropanol), C (0.02 g/L triphenylmethane dye, 10% v/v acetic acid), and D (10% v/v acetic acid), sequentially. Incubate and rock the gel in DI water overnight.
   5. Scan the gel. Convert the gel image to a grayscale image, followed by a black and white inversion and contrast enhancement to reveal bright protein bands in the black background.
   6. Measure the brightness of each band by summing the pixel values. Apply the linear fit C = aB + b, where B is the brightness of a tubulin band, C is the corresponding concentration, and a and b are fitting parameters. Determine the concentration of kinesin C_k by using the brightness of the kinesin band B_k to calculate the linear equation: C_k = aB_k + b.
4. Cluster the kinesin motors.
   1. Mix 1.5 µM kinesin (step 2.2.8) with 120 µM DTT and 120 nM streptavidin. Incubate on ice for 30 min.
   2. Store at -80 °C.

3. Prepare polyacrylamide-coated glass slides and coverslips (modified from Lau et al.⁴⁵)

1. Load new glass slides and glass coverslips in corresponding containers. Submerge the slides and coverslips in 1% v/v detergent in DI water and then boil the water in a microwave.
2. Sonicate the slides and coverslips for 5 min and then rinse with DI water to remove detergent.
3. Submerge and sonicate the slides and coverslips in ethanol for 5 min. Rinse with DI water.
4. Submerge and sonicate the slides and coverslips in 100 mM potassium hydroxide (KOH) for 5 min. Rinse with DI water.
5. Incubate the slides and coverslips in a silane solution (1% acetic acid and 0.5% 3-(trimethoxysilyl)propyl methacrylate in ethanol) for 15 min and then rinse with DI water.
6. Incubate the slides and coverslips in acrylamide solution (2% w/w acrylamide, 0.7 mg/mL ammonium persulfate, and 0.0035% v/v tetramethylethylenediamine in DI water) for ≥3 h.
7. Store the slides and coverslips in the acrylamide solution.

4. Prepare kinesin-driven, MT-based active fluids

1. Prepare active fluids (modified from Sanchez et al.¹⁹).
   NOTE: The following steps demonstrate the process of preparing 100 µL of an active fluid using example stocks. The final volume is scalable, and the concentrations of the example stocks can be adjusted as long as the final concentration of each component is maintained.
   1. Mix 16.7 µL of 8 mg/mL MTs (step 1.4.4) with 6.7 µL of 1.8 µM kinesin motor clusters (step 2.4.2), and 1.1 µL of 500 mM DTT in high-salt M2B (M2B + 3.9 mM MgCl₂).
   2. Bundle MTs by adding 11.4 µL of 7% w/w polyethylene glycol (PEG).
   3. Activate kinesin motors by adding 2.8 µL of 50 mM ATP.
   4. Maintain the ATP concentrations by adding 2.8 µL of stock pyruvate kinase/lactate dehydrogenase (PK/LDH) and 13.3 µL of 200 mM phosphenol pyruvate (PEP).
   5. Reduce the photobleaching effect by adding 10 µL of 20 mM Trolox, 1.1 µL of 3.5 mg/mL catalase, 1.1 µL of 20 mg/mL glucose oxidase, and 1.1 µL of 300 mg/mL glucose.
   6. Track the motion of the fluid by adding 1.6 µL of 0.025% v/v tracer particles.
   7. Add high-salt M2B to achieve a total volume of 100 µL.
      NOTE: The mixing orders in steps 4.1.1–4.1.6 are interchangeable. However, once ATP, MTs, and the motors are mixed, the motors start to consume ATP while stepping along the MTs. The sample is activated with a finite lifetime due to the limited fuels (ATP and PEP), so the experiment should be started promptly. The final active fluid should contain 1.3 mg/mL MT, 120 nM kinesin motor clusters, 5.5 mM DTT, 0.8% w/w PEG, 1.4 mM ATP, 2.8% v/v PK/LDH, 27 mM PEP, 2 mM Trolox, 0.038 mg/mL catalase, 0.22 mg/mL glucose oxidase, 3.3 mg/mL glucose, and 0.0004% v/v colloid in high-salt M2B.
2. Prepare a sample in a flow channel (modified from Chandrakar et al.⁴⁶):
   1. Rinse a polyacrylamide-coated glass slide and coverslip (step 3.7) with DI water. Dry the glasses with pressurized air. Place on a clean, flat surface.
   2. Cut two strips of wax films with a 3 mm width and the same length as the glass coverslip (20 mm). Insert the strips between the slide and coverslip as channel spacers.
   3. Adhere the glass to the wax film by placing the glass-wax complex on an 80 °C hot plate to melt the wax. During the melting, press the coverslip gently with a pipette tip to uniformly adhere the wax film to the glass surfaces. After adhesion, cool the glass complex to room temperature.
   4. Load the active fluids (step 4.1) to the flow channel. Seal the channel with UV glue.

5. Control sample temperature

1. Build a temperature control setup (modified from designs in Lowensohn et al. and Wu et al.^47,48,49).
   1. Prepare an aluminum cooling block.
      1. Mill an aluminum plate with dimensions of approximately 30 mm × 30 mm × 5 mm.
      2. Drill an internal channel through the plate (Figure 3A) and install hose fittings at the channel ends.
      3. Hook each fitting to a water tube.
      4. Connect one tube to a fish tank pump in a water reservoir while extending the other tube to the reservoir.
         NOTE: The pump will circulate reservoir water through the aluminum internal channels to maintain the block temperature at approximately room temperature.
   2. Wire a thermoelectric cooler (TEC) and a thermosensor to a temperature controller. Connect the controller to a computer using a USB port (Figure 3B).
   3. Wire the temperature controller to a direct current (DC) power supply. Turn on the controller by plugging the power supply to an electrical outlet.
   4. Perform the initial set up of the TEC following the controller manufacturer's guide. Test the TEC output. It is recommended to control the temperature controller via manufacturer-provided software, allowing thermosensor data recording and easier manipulation of the temperature controller.
   5. Identify the heating and cooling sides of the TEC.
   6. Attach the TEC's cooling side onto the cooling block (step 5.1.1) using thermal paste.
   7. Attach a sapphire disk to the TEC's heating side using thermal paste.
   8. The setup is complete. The sapphire surface and thermosensor can contact a sample to cool and heat the sample based on its temperature and target temperature.
      NOTE: It is recommended that the TEC and cooling block have an aligned central hole for imaging the samples using bright-field microscopy (Figure 3C).
2. Use the temperature control setup to control the sample temperature³³.
   1. Mount the sample to the setup.
      1. Place the active fluid sample (step 4.2.4) on the sapphire surface with the slide side contacting the surface.
      2. Secure the glass slide with paper tape.
      3. Attach the thermosensor to the coverslip surface using copper tape.
   2. Mount the setup on a microscope stage with the coverslip side facing toward the objectives. For example, on an inverted microscope, the coverslip side should face down. Secure the setup with paper tape and the microscope stage needle clamps if applicable.
      NOTE: The presented temperature stage should work with common microscopes that are either inverted or upright. To ensure that the temperature stage is not moved during the experiment, it is best to secure the temperature stage to the microscope stage with tape.
   3. Control the sample temperature.
      1. Turn on the temperature controller and fish tank pump.
      2. Follow the manufacturer's guide to set the target temperature and enable temperature control. The controller will adjust the heating or cooling power based on the target temperature and the sample temperature, as assessed by the thermosensor.
   4. Record sample temperatures.
      1. Follow the manufacturer's guide to record thermosensor temperature data during the experiment.
         NOTE: The sample temperature should now be controlled by the controller and recorded by the computer (Figure 3D).

6. Characterize the active fluid activity (modified from methods by Henkin et al. and Wu et al.^20,27)

NOTE: The previous sections are used to prepare active fluid samples (sections 1–4) and control their temperature (section 5). To demonstrate the use of temperature to control the active fluid activity, observe the fluid behaviors, analyze their activities, and characterize their response to temperature.

1. Monitor tracers.
   1. Image the sample with a constant interval Δt using green fluorescent protein (GFP) fluorescence to capture the movement of the tracer particles.
      NOTE: Δt should be chosen to allow the tracer movement to be tracked. Large Δt values, such as 100 s, result in losing the tracer trajectories, whereas short Δt values, such as 0.1 s, prevent the tracking algorithm from detecting the tracer movement between frames. A working Δt should allow the tracer displacement between frames to be within ~9 pixels. For imaging tracers moving at 10 µm/s using a 4x objective, the Δt is recommended to be 1–5 s.
   2. Save the images as TIFF files, name the files based on frame number, and store them in a separate folder. These processes are necessary to ensure that the acquired images will be correctly analyzed with the MATLAB script provided (step 6.2.2).
2. Track tracers adopting the tracking software developed by Ouellette et al.^50,51):
   1. Download the tracking software from the Environmental Complexity Laboratory, Stanford University (https://web.stanford.edu/~nto/software.shtml). Ensure that each MATLAB file is in the same folder.
   2. Track tracers using a custom MATLAB script: particle_tracking.m. The script reads tracer images (step 6.1) and tracks tracer movement using the software of Ouellette et al.^50,51. It outputs two files: 1) background.tif, representing the image background, and 2) Tracking.mat, containing the particle trajectories and velocities in each frame (Figure 4A).
3. Analyze the tracer mean speeds using a custom MATLAB script: analysis.m. The script reads the tracking file (Tracking.mat) and outputs the mean speed of tracers vs. time, along with a time-averaged mean speed with specified averaging windows (Figure 4B)³³.
4. Record the time-averaged mean speed.
5. Measure the time-averaged mean speed by repeating the experiment (steps 4, 5.2 and 6.1–6.4) at 10–40 °C. Use the recorded mean speeds to plot the mean speed vs. temperature (Figure 4C)³³.

Results

Preparing the kinesin-driven, MT-based active fluids requires both kinesin and MTs. The MTs were polymerized from labeled tubulins (steps 1.3 and 1.4) that were purified from bovine brains (step 1.1, Figure 2A), followed by recycling to enhance purity (step 1.2, Figure 2B). The kinesin motor proteins were expressed in and purified from E. coli (steps 2.1 and 2.2, Figure 2B)...

Discussion

Controlling active matter in situ opens the door to directed self-organization of active matter^4,5,24,28,54. In this article, we present a protocol for using temperature to control kinesin-driven, MT-based active fluids in situ, based on the Arrhenius characteristic of the system^29,30,

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid	Sigma-Aldrich	238813	Trolox
2-Mercaptoethanol	Sigma-Aldrich	M6250
3-(Trimethoxysilyl)propyl methacrylate, 98%, ACROS Organics	Fisher Scientific	AC216550050
3.2mm I.D. Tygon Tubing R-3603	HACH	2074038	Water tubes
31.75 mm diameter uncoated, sapphire window	Edmund Optics	43-637	Sapphire disc
3M 1181 Copper Tape - 1/2 IN Width X 18 YD Length - 2.6 MIL Total Thickness - 27551	R.S. HUGHES	054007-27551	Copper tape
Acetic Acid	Sigma-Aldrich	A6283
Acrylamide Solution (40%/Electrophoresis), Fisher BioReagents	Fisher Scientific	BP1402-1
Adenosine 5'-triphosphate dipotassium salt hydrate	Sigma-Aldrich	A8937	ATP
Alexa Fluor 647 NHS Ester (Succinimidyl Ester)	Thermo Fisher Scientific	A20006	Far-red fluorescent dye. Alexa 647 can be pre suspended in dimethylsulfoxide (DMSO) before mixing with microtubules (1.3.3.2.)
Amicon Ultra-4 Centrifugal Filter Unit	Sigma-Aldrich	UFC801024	Centrifugal filter tube. Cutoff molecular weight: 10 kDa
Ammonium Persulfate, 100g, MP Biomedicals	Fisher Scientific	ICN802829	APS
Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents	Fisher Scientific	BP1760	Ampicillin
Antivibration Table	Nikon	63-7590S
Avanti J-E Centrifuge	Beckman Coulter	369001
Bacto Agar Soldifying Agent, BD Diagnostics	VWR	90000-760	Agar
Biotin	Alfa Aesar	A14207
Bucket-plastic white - 2 gallon	Bon	84-715	Water bucket
Calcium Chloride	Sigma-Aldrich	746495	CaCl2
Catalase from bovine liver	Sigma-Aldrich	C40
CFI Plan Apo Lambda 4x Obj	Nikon	MRD00045	4x air objective
C-FLLL-FOV GFP HC HC HISN ero Shift	Nikon	96372	GFP filter cube
CH-109-1.4-1.5	TE Technology	CH-109-1.4-1.5	Thermoelectric Cooler (TEC)
Chloramphenicol, 98%, ACROS Organics	Fisher Scientific	C0378
Cooling block	N/A	N/A	Custom milled aluminum
Coomassie Brilliant Blue R-250 #1610400	Bio-Rad	1610400	Triphenylmethane dye
D-(+)-Glucose	Sigma-Aldrich	G7528
Dimethyl Sulfoxide (Certified ACS), Fisher Chemical	Fisher Scientific	D128	DMSO
DL-1,4-Dithiothreitol, 99%, for biochemistry, ACROS Organics	Fisher Scientific	AC165680050	DTT
DOWSIL 340 Heat Sink Compound	Dow	1446622	Thermal paste
ETHYL ALCOHOL, 200 PROOF ACS/USP/NF GRADE 5 GALLON POLY CUBE	Pharmco by Greenfield Global	111000200CB05	Ethanol
Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid	Sigma-Aldrich	E3889	EGTA
Ethylenediaminetetraacetic acid	Sigma-Aldrich	798681	EDTA
Fisher BioReagents Microbiology Media Additives: Tryptone	Fisher Scientific	BP1421	Tryptone
Fisher BioReagents Microbiology Media Additives: Yeast Extract	Fisher Scientific	BP1422	Yeast extract
Fluoresbrite YG Microspheres, Calibration Grade 3.00 µm	Polysciences	18861	Tracer particles
Glucose Oxidase from Aspergillus niger	Sigma-Aldrich	G2133
Glycerol	Sigma-Aldrich	G5516
GpCpp	Jena Bioscience	NU-405L	Guanosine-5’[(α,β)-methyleno]triphosphate (GMPCPP)
GS Power's 18 Gauge (True American Wire Ga), 100 feet, 99.9% Stranded Oxygen Free Copper OFC, Red/Black 2 Conductor Bonded Zip Cord Power/Speaker Electrical Cable for Car, Audio, Home Theater	Amazon	B07428NBCW	Copper wire
Guanosine 5'-triphosphate sodium salt hydrate	Sigma-Aldrich	G8877	GTP
Hellmanex III	Sigma-Aldrich	Z805939	Detergent
HEPES Sodium Salt (White Powder), Fisher BioReagents	Fisher Scientific	BP410	NaHEPES
High performance blender machine	AIMORES	AS-UP1250	Blender
His GraviTrap	GE Healthcare	11003399	Gravity Column
Imidazole	Sigma-Aldrich	I5513
IPTG	Sigma-Aldrich	I6758	Isopropyl β-D-1-thiogalactopyranoside
Isopropyl Alcohol 99%	Pharmco by Greenfield Global	231000099	Isopropanol
JA-10 rotor	Beckman Coulter	369687
L-Glutamic acid potassium salt monohydrate	Sigma-Aldrich	G1501	K-Glutamate
Lysozyme from chicken egg white	Sigma-Aldrich	L6876
Magnesium chloride hexahydrate	Sigma-Aldrich	M2670	MgCl2•6H2O
MES sodium salt	Sigma-Aldrich	M5057	2-(N-Morpholino)ethanesulfonic acid sodium salt
MOPS	Sigma-Aldrich	M1254	3-(N-Morpholino)propanesulfonic acid
MP-3022	TE Technology	MP-3022	Thermocouple
N,N,N',N'-Tetramethylethylenediamine 99%, ACROS Organics	Fisher Scientific	AC138450500	TEMED
Nanodrop 2000c UV-VIS Spectrophotometer	Thermo Fisher Scientific	E112352	Spectrometer
Nikon Ti2-E Nikon Inverted Microscope	Nikon	MEA54000
Norland Optical Adhesive 81	Norland Products	NOA81	UV glue
Novex Sharp Pre-stained Protein Standard	Thermo Fisher Scientific	LC5800	Protein standard ladder
NuPAGE 4-12% Bis-Tris Protein Gels, 1.5 mm, 10-well	Thermo Fisher Scientific	NP0335BOX	SDS gel
Optima L-90K Ultracentrifuge	Beckman Coulter	365672
Parafilm PM996 Wrap, 4" Wide; 125 Ft/Roll	Cole-Parmer	EW-06720-40	Wax film
Pe 300 ultra Illumination System Single Band, 3mm Light Guide control Pod power supply	Nikon	PE-300-UT-L-SB-40	Cool LED Illuminator
Phenylmethanesulfonyl fluoride	Sigma-Aldrich	78830	PMSF
Phosphoenolpyruvic acid monopotassium salt, 99%	BeanTown Chemical	129745	PEP
Pierce Coomassie (Bradford) Protein Assay Kit	Thermo Fisher Scientific	23200
Pierce Protease Inhibitor Mini Tablets	Thermo Fisher Scientific	A32953
PIPES	Sigma-Aldrich	P6757	1,4-Piperazinediethanesulfonic acid
Pluronic F-127	Sigma-Aldrich	P2443
Poly(ethylene glycol)	Sigma-Aldrich	81300	PEG. Average molecular weight 20,000 Da
Potassium Hydroxide (Pellets/Certified ACS), Fisher Chemical	Fisher Scientific	P250-500	KOH
PowerEase 300W Power Supply (115 VAC)	ThermoFisher Scientific	PS0300	DC power supply of the gel box
PS-12-8.4A	TE Technology	PS-12-8.4A	DC power supply of the temperature controller
Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle	Sigma-Aldrich	P-0294	PK/LDH
Quiet One Lifegard Fountain Pump, 296-Gallon Per Hour	Amazon	B005JWA612	Fish tank pump
Rosetta 2(DE3)pLysS Competent Cells - Novagen	Millipore Sigma	71403	Competent cells
Sharp Microwave ZSMC0912BS Sharp 900W Countertop Microwave Oven, 0.9 Cubic Foot, Stainless Steel	Amazon	B01MT6JZMR	Microwave for boiling the water
Sodium Chloride (Crystalline/Certified ACS), Fisher Chemical	Fisher Scientific	S271-500	NaCl
Sodium dodecyl sulfate	Sigma-Aldrich	L3771	SDS
Sodium phosphate monobasic	Sigma-Aldrich	S8282	NaH2PO4
Streptavidin Protein	Thermo Fisher Scientific	21122
Sucrose	Sigma-Aldrich	S7903
TC-720	TE Technology	TC-720	Temperature controller
Tris Base, Molecular Biology Grade - CAS 77-86-1 - Calbiochem	Sigma-Aldrich	648310	Tris-HCL
Type 45 Ti rotor	Beckman Coulter	339160
Type 70 Ti rotor	Beckman Coulter	337922
Type 70.1 Ti rotor	Beckman Coulter	342184
VWR General-Purpose Laboratory Labeling Tape	VWR	89097-916	Paper tapes
VWR Micro Cover Glasses, Square, No. 1 1/2	VWR	48366-227	Glass coverslips
VWR Plain and Frosted Micro Slides, Premium	VWR	75799-268	Glass slides
XCell SureLock Mini-Cell	ThermoFisher Scientific	EI0001	Gel box
ZYLA 5.5 USB3.0 Camera	Nikon	ZYLA5.5-USB3	Monochrome CCD camera