Erratum: Noninvasive and Invasive Renal Hypoxia Monitoring in a Porcine Model of Hemorrhagic Shock

Summary

An erratum was issued for: Noninvasive and Invasive Renal Hypoxia Monitoring in a Porcine Model of Hemorrhagic Shock. The Protocol and Table of Materials were updated.

Abstract

An erratum was issued for: Noninvasive and Invasive Renal Hypoxia Monitoring in a Porcine Model of Hemorrhagic Shock. The Protocol section was updated.

Step 2.3.1 - 2.3.1.2 of the Protocol was updated from:

1. Measure PuO₂ in the bladder.
   1. Remove all air from the bladder by slowly squeezing the bladder while ensuring urine does not leak out.
   2. Place the tip of the luminescence quenching-based PuO₂ sensor in the bladder via a cystotomy, similar to the catheter.
   3. Connect the fiber optic cable from the bladder sensor to the data collection device.

to:

1.  Measure PuO₂ at the outlet of the bladder
   1. Identify the balloon on the catheter. Just below the balloon make an incision along the long axis of the catheter, ensuring that you do not cut the lumen that connects to the balloon. 
   2. After making the incision, insert a t-connector that contains the sensing material into the incision. 
   3. Use tissue glue to secure the t-connector in place. 
   4. Connect the fiber optic cable from the bladder data collection device to the connector that contains the sensing material. 

Step 2.3.2.2 - 2.3.2.7 of the Protocol was updated from:

2. Make a flank incision large enough to expose the kidney (approx. 2-3 in) on the side of the pig at approximately the same location where the kidney was identified.
3. With the tips of a retractor together, introduce the retractor into the incision and then spread the tips of the retractor to expose the kidney.
4. Use a micro-manipulator or similar tool to hold the oxygen probe steady. If possible, attach this tool to the end of an articulating arm.
5. Attach the other end of the articulating arm to the surgical table so that the other end that will hold the oxygen probe is near the opened incision. If the tool that is used to hold the oxygen probe is not connected to an articulating arm, position the tool so the oxygen sensor is near the opened incision and is stable.
6. Unlock all articulating joints of the arm. Using ultrasound, place the tip of the oxygen probe in the medulla region of the kidney. Lock all articulating joints on the arm.
7. After confirming placement of the tip of the sensor in the medulla with ultrasound, use the micromanipulator to retract the needle housing the luminescence-based oxygen sensor. Connect the other end of the sensor to the data collection device connected to the computer running the data collection software. Start recording.

to:

2. Move the bowel so that you have a clear line of site and access to the entire kidney. 
3. Insert the sensor into 2" 18 gauge catheter. Adjust the luer lock connector on the sensor so that the tip of the sensor is exposed. Remove the catheter and place it over an 18 gauge needle.
4. Place the 18 gauge needle and 2 in catheter into the renal medulla under ultrasound guidance.
5. Remove the needle, keeping the catheter in place. Thread the tissue sensor through the catheter and use the luer lock to connect the sensor to the catheter. 
6. Use tissue glue to secure the catheter in place. 
7. Connect the tissue sensor to the data collection box.

The Table of Materials was updated from:

Name	Company	Catalog Number	Comments
1/8" PVC tubing	Qosina	SKU: T4307	Part of noninvasive PuO2 monitor
3/16" PVC tubing	Qosina	SKU: T4310	Part of noninvasive PuO2 monitor
3/32" (1), 1/8" (1), 5/32" (1) drill bit	Dewalt	N/A	For building noninvasive PuO2 monitor
3/8" TPE tubing	Qosina	SKU: T2204	Part of noninvasive PuO2 monitor
Biocompatible Glue	Masterbond	EP30MED	Part of noninvasive PuO2 monitor
Bladder oxygen measurement device	Presens	Fibox 4	Stand-alone fiber optic oxygen meter
Bladder PuO2 sensor	Presens	DP-PSt3	Oxygen dipping probe
Chlorhexidine 4% scrub	Vetone	N/A	For scrubbing insertion or puncture sites
Conical connector with female luer lock	Qosina	SKU: 51500	Part of noninvasive PuO2 monitor
Cuffed endotracheal tube	Vetone	600508	For sedating the subject and providing respiratory support
Euthanasia solution (pentobarbital sodium|pheyntoin sodium)	Vetone	11168	For euthanasia after completion of experiment
General purpose temperature probe, 400 series thermistor	Novamed	10-1610-040	Part of noninvasive PuO2 monitor
Hemmtop Magic Arm 11 inch	Amazon	B08JTZRKYN	Holding invasive oxygen sensor in place
HotDog veterinary warming system	HotDog	V106	For controlling subject temperature during experiment
Invasive tissue oxygen measurement device	Presens	Oxy-1 ST	Compact oxygen transmitter
Invasive tissue oxygen sensor	Presens	PM-PSt7	Profiling oxygen microsensor
Isoflurane	Vetone	501017	To maintain sedation throughout the experiment
Isotonic crystalloid solution	HenrySchein	1537930 or 1534612	Used during resuscitation in the critical care period
Liquid flow sensor	Sensirion	LD20-2600B	Part of noninvasive PuO2 monitor
Male luer lock to barb connector	Qosina	SKU: 11549	Part of noninvasive PuO2 monitor
Male to male luer connector	Qosina	SKU: 20024	Part of noninvasive PuO2 monitor
Noninvasive oxygen measurement device	Presens	EOM-O2-mini	Electro optical module transmitter for contactless oxygen measurements
Non-vented male luer lock cap	Qosina	SKU: 65418	Part of noninvasive PuO2 monitor
Norepinephrine	HenrySchein	AIN00610	Infusion during resuscitation
O2 sensor stick	Presens	SST-PSt3-YOP	Part of noninvasive PuO2 monitor
PowerLab data acquisition platform	AD Instruments	N/A	For data collection
REBOA catheter	Certus Critical Care	N/A	Used in experimental protocol
Super Sheath arterial catheters (5 Fr, 7 Fr, 9 Fr)	Boston Scientific	C1894	For intravascular access
Suture	Ethicon	C013D	For securing catheter to skin and closing incisions
T connector, all female luer locks	Qosina	SKU: 88214	Part of noninvasive PuO2 monitor

to:

Name	Company	Catalog Number	Comments
1/8" PVC tubing	Qosina	SKU: T4307	Part of noninvasive PuO2 monitor
3/16" PVC tubing	Qosina	SKU: T4310	Part of noninvasive PuO2 monitor
3/8" TPE tubing	Qosina	SKU: T2204	Part of noninvasive PuO2 monitor
3/32" (1), 1/8" (1), 5/32" (1) drill bit	Dewalt	N/A	For building noninvasive PuO2 monitor
Biocompatible Glue	Masterbond	EP30MED	Part of noninvasive PuO2 monitor
Bladder PuO2 sensor	Presens	DP-PSt3	Oxygen dipping probe
Bladder oxygen measurement device	Presens	Fibox 4	Stand-alone fiber optic oxygen meter
Chlorhexidine 4% scrub	Vetone	N/A	For scrubbing insertion or puncture sites
Conical connector with female luer lock	Qosina	SKU: 51500	Part of noninvasive PuO2 monitor
Cuffed endotracheal tube	Vetone	600508	For sedating the subject and providing respiratory support
Euthanasia solution (pentobarbital sodium|pheyntoin sodium)	Vetone	11168	For euthanasia after completion of experiment
General purpose temperature probe, 400 series thermistor	Novamed	10-1610-040	Part of noninvasive PuO2 monitor
HotDog veterinary warming system	HotDog	V106	For controlling subject temperature during experiment
Invasive tissue oxygen measurement device	Optronix	N/A	OxyLite™ oxygen monitors
Invasive tissue oxygen sensor	Optronix	NX-BF/OT/E	Oxygen/Temperature bare-fibre sensor
Isoflurane	Vetone	501017	To maintain sedation throughout the experiment
Isotonic crystalloid solution	HenrySchein	1537930 or 1534612	Used during resuscitation in the critical care period
Liquid flow sensor	Sensirion	LD20-2600B	Part of noninvasive PuO2 monitor
Male luer lock to barb connector	Qosina	SKU: 11549	Part of noninvasive PuO2 monitor
Male to male luer connector	Qosina	SKU: 20024	Part of noninvasive PuO2 monitor
Norepinephrine	HenrySchein	AIN00610	Infusion during resuscitation
Noninvasive oxygen measurement device	Presens	EOM-O2-mini	Electro optical module transmitter for contactless oxygen measurements
Non-vented male luer lock cap	Qosina	SKU: 65418	Part of noninvasive PuO2 monitor
O2 sensor stick	Presens	SST-PSt3-YOP	Part of noninvasive PuO2 monitor
PowerLab data acquisition platform	AD Instruments	N/A	For data collection
REBOA catheter	Certus Critical Care	N/A	Used in experimental protocol
Super Sheath arterial catheters (5 Fr, 7 Fr, 9 Fr)	Boston Scientific	C1894	for intravascular access
Suture	Ethicon	C013D	For securing catheter to skin and closing incisions
T connector, all female luer locks	Qosina	SKU: 88214	Part of noninvasive PuO2 monitor

Protocol

An erratum was issued for: Noninvasive and Invasive Renal Hypoxia Monitoring in a Porcine Model of Hemorrhagic Shock. The Protocol section was updated.

Step 2.3.1 - 2.3.1.2 of the Protocol was updated from:

1. Measure PuO₂ in the bladder.
   1. Remove all air from the bladder by slowly squeezing the bladder while ensuring urine does not leak out.
   2. Place the tip of the luminescence quenching-based PuO₂ sensor in the bladder via a cystotomy, similar to the catheter.
   3. Connect the fiber optic cable from the bladder sensor to the data collection device.

to:

1.  Measure PuO₂ at the outlet of the bladder
   1. Identify the balloon on the catheter. Just below the balloon make an incision along the long axis of the catheter, ensuring that you do not cut the lumen that connects to the balloon. 
   2. After making the incision, insert a t-connector that contains the sensing material into the incision. 
   3. Use tissue glue to secure the t-connector in place. 
   4. Connect the fiber optic cable from the bladder data collection device to the connector that contains the sensing material. 

Step 2.3.2.2 - 2.3.2.7 of the Protocol was updated from:

2. Make a flank incision large enough to expose the kidney (approx. 2-3 in) on the side of the pig at approximately the same location where the kidney was identified.
3. With the tips of a retractor together, introduce the retractor into the incision and then spread the tips of the retractor to expose the kidney.
4. Use a micro-manipulator or similar tool to hold the oxygen probe steady. If possible, attach this tool to the end of an articulating arm.
5. Attach the other end of the articulating arm to the surgical table so that the other end that will hold the oxygen probe is near the opened incision. If the tool that is used to hold the oxygen probe is not connected to an articulating arm, position the tool so the oxygen sensor is near the opened incision and is stable.
6. Unlock all articulating joints of the arm. Using ultrasound, place the tip of the oxygen probe in the medulla region of the kidney. Lock all articulating joints on the arm.
7. After confirming placement of the tip of the sensor in the medulla with ultrasound, use the micromanipulator to retract the needle housing the luminescence-based oxygen sensor. Connect the other end of the sensor to the data collection device connected to the computer running the data collection software. Start recording.

to:

2. Move the bowel so that you have a clear line of site and access to the entire kidney. 
3. Insert the sensor into 2" 18 gauge catheter. Adjust the luer lock connector on the sensor so that the tip of the sensor is exposed. Remove the catheter and place it over an 18 gauge needle.
4. Place the 18 gauge needle and 2 in catheter into the renal medulla under ultrasound guidance.
5. Remove the needle, keeping the catheter in place. Thread the tissue sensor through the catheter and use the luer lock to connect the sensor to the catheter. 
6. Use tissue glue to secure the catheter in place. 
7. Connect the tissue sensor to the data collection box.

The Table of Materials was updated from:

Name	Company	Catalog Number	Comments
1/8" PVC tubing	Qosina	SKU: T4307	Part of noninvasive PuO2 monitor
3/16" PVC tubing	Qosina	SKU: T4310	Part of noninvasive PuO2 monitor
3/32" (1), 1/8" (1), 5/32" (1) drill bit	Dewalt	N/A	For building noninvasive PuO2 monitor
3/8" TPE tubing	Qosina	SKU: T2204	Part of noninvasive PuO2 monitor
Biocompatible Glue	Masterbond	EP30MED	Part of noninvasive PuO2 monitor
Bladder oxygen measurement device	Presens	Fibox 4	Stand-alone fiber optic oxygen meter
Bladder PuO2 sensor	Presens	DP-PSt3	Oxygen dipping probe
Chlorhexidine 4% scrub	Vetone	N/A	For scrubbing insertion or puncture sites
Conical connector with female luer lock	Qosina	SKU: 51500	Part of noninvasive PuO2 monitor
Cuffed endotracheal tube	Vetone	600508	For sedating the subject and providing respiratory support
Euthanasia solution (pentobarbital sodium|pheyntoin sodium)	Vetone	11168	For euthanasia after completion of experiment
General purpose temperature probe, 400 series thermistor	Novamed	10-1610-040	Part of noninvasive PuO2 monitor
Hemmtop Magic Arm 11 inch	Amazon	B08JTZRKYN	Holding invasive oxygen sensor in place
HotDog veterinary warming system	HotDog	V106	For controlling subject temperature during experiment
Invasive tissue oxygen measurement device	Presens	Oxy-1 ST	Compact oxygen transmitter
Invasive tissue oxygen sensor	Presens	PM-PSt7	Profiling oxygen microsensor
Isoflurane	Vetone	501017	To maintain sedation throughout the experiment
Isotonic crystalloid solution	HenrySchein	1537930 or 1534612	Used during resuscitation in the critical care period
Liquid flow sensor	Sensirion	LD20-2600B	Part of noninvasive PuO2 monitor
Male luer lock to barb connector	Qosina	SKU: 11549	Part of noninvasive PuO2 monitor
Male to male luer connector	Qosina	SKU: 20024	Part of noninvasive PuO2 monitor
Noninvasive oxygen measurement device	Presens	EOM-O2-mini	Electro optical module transmitter for contactless oxygen measurements
Non-vented male luer lock cap	Qosina	SKU: 65418	Part of noninvasive PuO2 monitor
Norepinephrine	HenrySchein	AIN00610	Infusion during resuscitation
O2 sensor stick	Presens	SST-PSt3-YOP	Part of noninvasive PuO2 monitor
PowerLab data acquisition platform	AD Instruments	N/A	For data collection
REBOA catheter	Certus Critical Care	N/A	Used in experimental protocol
Super Sheath arterial catheters (5 Fr, 7 Fr, 9 Fr)	Boston Scientific	C1894	For intravascular access
Suture	Ethicon	C013D	For securing catheter to skin and closing incisions
T connector, all female luer locks	Qosina	SKU: 88214	Part of noninvasive PuO2 monitor

to:

Name	Company	Catalog Number	Comments
1/8" PVC tubing	Qosina	SKU: T4307	Part of noninvasive PuO2 monitor
3/16" PVC tubing	Qosina	SKU: T4310	Part of noninvasive PuO2 monitor
3/8" TPE tubing	Qosina	SKU: T2204	Part of noninvasive PuO2 monitor
3/32" (1), 1/8" (1), 5/32" (1) drill bit	Dewalt	N/A	For building noninvasive PuO2 monitor
Biocompatible Glue	Masterbond	EP30MED	Part of noninvasive PuO2 monitor
Bladder PuO2 sensor	Presens	DP-PSt3	Oxygen dipping probe
Bladder oxygen measurement device	Presens	Fibox 4	Stand-alone fiber optic oxygen meter
Chlorhexidine 4% scrub	Vetone	N/A	For scrubbing insertion or puncture sites
Conical connector with female luer lock	Qosina	SKU: 51500	Part of noninvasive PuO2 monitor
Cuffed endotracheal tube	Vetone	600508	For sedating the subject and providing respiratory support
Euthanasia solution (pentobarbital sodium|pheyntoin sodium)	Vetone	11168	For euthanasia after completion of experiment
General purpose temperature probe, 400 series thermistor	Novamed	10-1610-040	Part of noninvasive PuO2 monitor
HotDog veterinary warming system	HotDog	V106	For controlling subject temperature during experiment
Invasive tissue oxygen measurement device	Optronix	N/A	OxyLite™ oxygen monitors
Invasive tissue oxygen sensor	Optronix	NX-BF/OT/E	Oxygen/Temperature bare-fibre sensor
Isoflurane	Vetone	501017	To maintain sedation throughout the experiment
Isotonic crystalloid solution	HenrySchein	1537930 or 1534612	Used during resuscitation in the critical care period
Liquid flow sensor	Sensirion	LD20-2600B	Part of noninvasive PuO2 monitor
Male luer lock to barb connector	Qosina	SKU: 11549	Part of noninvasive PuO2 monitor
Male to male luer connector	Qosina	SKU: 20024	Part of noninvasive PuO2 monitor
Norepinephrine	HenrySchein	AIN00610	Infusion during resuscitation
Noninvasive oxygen measurement device	Presens	EOM-O2-mini	Electro optical module transmitter for contactless oxygen measurements
Non-vented male luer lock cap	Qosina	SKU: 65418	Part of noninvasive PuO2 monitor
O2 sensor stick	Presens	SST-PSt3-YOP	Part of noninvasive PuO2 monitor
PowerLab data acquisition platform	AD Instruments	N/A	For data collection
REBOA catheter	Certus Critical Care	N/A	Used in experimental protocol
Super Sheath arterial catheters (5 Fr, 7 Fr, 9 Fr)	Boston Scientific	C1894	for intravascular access
Suture	Ethicon	C013D	For securing catheter to skin and closing incisions
T connector, all female luer locks	Qosina	SKU: 88214	Part of noninvasive PuO2 monitor