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The current protocol contains a novel component that might be critical to tackling current obstacles to incorporating eye-tracking in visual duration perception tasks. The critical step here is the definition of time windows based on cognitive processes that putatively take place in each of these time windows. In the system we used, time windows can only be defined as Areas of Interest (a space-related concept that is coupled with time in these systems), but in other systems, it is possible to do this by exporting different segments of the trial. In addition to this temporal segmentation of the trial, it is important to focus on analyzing changes across time windows rather than the parameters per time window.
Concerning the modifications to the protocol that had to be made, they were mostly related to the dimensions of the area of interest. We made a first attempt using dynamic AOIs - defining a spatial selection around the stimulus that followed it, rather than the whole screen. However, we soon realized that we could be missing relevant events outside that area. Given that our measures were unrelated to focus on the stimulus (pupil size was expected to change according to cognitive load and not according to attention to the flash or ball; the number of fixations was expected to reflect spatial search), we chose to use the full screen as the region of interest.
The current protocol is an embryonic proposal that is still subject to many refinements. We will only highlight two of these, even though there is much more room for improvement. The first concerns the differences in the length of the three time windows, which preclude us from interpreting time window effects on the number of fixations (e.g., a longer time window entails more fixations, hence the decrease from TW0 to TW1, see Figure 3). One way of addressing this problem would be to consider the number of fixations per time unit.
The second relates to the correspondence between time windows and putative ongoing processes, which includes various issues. One is that TW1 does not represent just stimulus appearance but probably also an explicit form of interval estimation (first interval) subsidiary to interval comparison and likely absent in TW0. In a similar fashion, changes across time windows may also reflect changes in general processes such as sustained attention and working memory18, even though some of these changes could be expected in an interval comparison task (working memory load is expected to increase from TW1 over TW2). One way to attenuate these potential confounds would be to introduce control tasks related to pure duration estimation, sustained attention and working memory, and then base the eye-tracking data analysis on the comparison between experimental (interval comparison) and control tasks. Another issue is that the duration of TW0 was irrelevant to the task, and it is known that task-irrelevant durations may be deleterious to performance19. Future work could focus on improving this, namely by creating a difference of 300 ms between TW0 (irrelevant interval) and TW1 to better delimit visual processing responses, since a short event can be biased to be perceived earlier or later than its presentation by simply adding another event in near temporal proximity20,21.
Finally, spontaneous eye blinks can affect time perception by distorting it (dilating time if an eye blink precedes the interval, contracting if it occurs simultaneously), potentially introducing variability in intra-individual timing performance22. One way of minimizing this problem would be to apply an eye-blink-based correction factor in participants' behavioral judgments (e.g., assign a reliability rate to each judgment depending on the presence of blinks before or during the stimuli. Additionally, incorporating the statistical approach of treating trials as random variables may also aid in addressing this problem.
Regarding future research, an important topic to address would be the association between spontaneous eye blink rate (EBR) and time perception. EBR has been known to be a non-invasive indirect marker of central dopamine function (DA)23, and, more recently, high ERB was associated with poorer temporal perception. The study suggests an implication of dopamine in interval timing and points to the use of ERB as a proxy of dopamine measure24. Another important topic is the functional meaning of the (change-related) measures we analyzed, which is yet to be determined in the context of our paradigm. In the original study, as well as in the current simplified dataset, increases in pupil size from TW0 to TW1 were consistent with the idea of increased cognitive load, but the absence of group effects on this measure precludes further considerations. One pattern that seems to present is that smaller changes across time windows correlated with better behavioral performance (Flashes better than Balls, and d-prime in dyslexics related to smaller changes), but further research is needed.
Despite its limitations, the current protocol is, to our knowledge, the first to show parallel results in eye-tracking and behavioral data (same profile of effects), as well as some evidence of the correlation between the two.