Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Biology

Monitoring Actin Disassembly with Time-lapse Microscopy

doi: 10.3791/66 Published: November 8, 2006

Protocol

or Start trial to access full content. Learn more about your institution’s access to JoVE content here
  1. Construct a flow chamber as shown in the video.  Make sure that the parafilm seal is tight and use washed coverslips.
  2. Incubate actin-binding agent in the chamber for 5-10'.
  3. Block non-specific binding sites on the glass coverslip with a blocking protein.
  4. Polymerize actin inside the chamber by flowing in G-actin in polymerizing buffer.
  5. Washout unpolymerized actin by flowing in excess buffer.

Subscription Required. Please recommend JoVE to your librarian.

Monitoring Actin Disassembly with Time-lapse Microscopy
Play Video
PDF DOI

Cite this Article

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).More

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter