Glue-Based Tissue Microarray Construction for Tumor and Disease Research

Summary

This protocol describes a low-cost production and efficient validation method for glue-based TMA construction, providing a convenient pathological diagnosis platform for tumor and disease research.

Abstract

Tissue microarray technology (TMA) is a high-throughput platform for the simultaneous detection and analysis of multiple tissue samples, facilitating efficient tumor and disease biomarker research. However, conventional TMA construction methods often face limitations such as operational complexity, time-consuming procedures, and variable accuracy. A glue-based TMA construction method was developed to overcome these challenges, offering improved tissue core fixation and enhanced structural stability. Systematic validation included histological evaluation (HE staining), immunohistochemical profiling of target proteins, and fluorescence in situ hybridization (FISH) analysis. Results demonstrated that the glue-based method maintained excellent slice integrity, improved signal-to-noise ratio, and ensured consistent batch-to-batch reproducibility. Although the approach is limited to manual operation, it presents a reliable and cost-effective option for moderate-throughput TMA production. This method is particularly suited to research environments that value flexibility and sample diversity over large-scale automation, expanding the utility of TMA in academic and diagnostic settings.

Introduction

Tissue Microarray (TMA) is a high-throughput technique for analyzing tissue samples^1,2,3. Multiple donor tissue cores are obtained, and donor paraffin blocks are transferred into recipient tissue blocks for simultaneous differential and comparative molecular analysis under theoretically the same performance conditions^2,4. The classic way to construct a tissue microarray (TMA) is to use a hole punch to extract tissue cores from a donor tissue sample and arrange them sequentially into a recipient paraffin block. This method is suitable for donor paraffin blocks of similar depth and allows for the efficient analysis of multiple samples on a single slice, greatly improving the efficiency of the study and the consistency of data^5,6,7. Nevertheless, this method still possesses certain limitations, including inadequate handling of the tissue core during the embedding process, which may result in inconsistent staining of samples in subsequent analyses⁸.

The second method of TMA construction is the tape method^9,10. This method inverts the construction process by casting the block around inverted upright cores that, upon completion, are flush with the top of the TMA, irrespective of core length^11,12. However, this method requires ensuring the proper placement of the tissue core and the effectiveness of the tape, and also limits the number of samples that can be processed compared to traditional techniques.

This study proposes an innovative glue method for constructing TMA, aiming to solve problems such as the insufficient stability of tissue cores and complex operation that exist in traditional techniques¹³. This method uses glue to precisely fix multiple tissue cores together and has the advantages of simple operation and strong sample firmness. Compared with the traditional sectioning or tape methods, the glue method can increase the retention rate of tissue cores and reduce costs. This method is applicable to clinical studies with a medium sample size and is particularly suitable for research designs that require flexible adjustment of the experimental plan. However, it should be noted that the processing capacity of this method does not meet the demands of ultra-high throughput⁹. Meanwhile, in the actual application process, a complete set of standardized operation norms and quality control systems must be established. Operators need to receive systematic training and pass professional assessments to ensure the standardization of technical operations and the repeatability of results. Comprehensive analysis indicates that this technology achieves a good balance among operational flexibility, cost-effectiveness, and technical reliability, and is particularly suitable for research scenarios where resources are limited but quality control still needs to be guaranteed.

Protocol

All donor blocks were obtained from archival pathological specimens collected between 2016 and 2018 at the Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University. The samples were deidentified prior to use and processed in compliance with approved protocols (the ethics committee of the Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, KY-2024-250-01).

1. Assessment and tagging of donor tissue

1. Select tissue blocks with a thickness of ≥5 mm and an area of ≥15 mm × 15 mm. Ensure a 2 mm safety margin at the edges, and exclude regions exhibiting necrosis, hemorrhage, or calcification to maintain tissue integrity.
2. Target area identification and marking
   1. Evaluate the H&E-stained sections using an optical microscope. Locate the target area through low-power scanning, then switch to high-power to confirm the cellular morphological characteristics. Mark the target area boundaries on the slides.
   2. Map the coordinates of the selected area to the surface of the paraffin block to complete the marking conversion.

2. Tissue core extraction

1. Puncture preparation and sample pretreatment
   1. Select a 2 mm diameter hand-held hole puncher and confirm the smoothness of its cutting edge and ease of operation.
   2. Place the donor paraffin block on a cold platform at 4 °C for 15 min.
2. Align the needle vertically with the marked point, and apply steady rotational pressure to reach the limiting depth. Avoid tilting or vibrating during the process⁷ (Figure 1A).
3. Gently rotate the needle counterclockwise (≤2 rotations/s) to withdraw it. Carefully eject the tissue core from the needle and transfer it into an individual well of a pre-chilled 96-well plate. Record the core location in each well (Figure 1B).
   NOTE: If the bottom of the tissue core is uneven, level it with a blade. After trimming, recheck the integrity.
4. Pre-record all donor numbers and the corresponding ELISA plate well positions (A1-H12) in the registration form. During the operation, recheck to ensure strict correspondence between donor numbers and well positions.
5. Immediately inspect the cores for integrity. Discard cores that are fractured, bent, or exhibit diameter deviations >0.1 mm.
   NOTE: Ensure core length consistency (coefficient of variation, CV ≤5%) and avoid surface scratches or compression artifacts.

3. Tissue core fixation

1. Use a waterproof marker to draw a grid directly on the mold surface, ensuring clear and evenly spaced lines.
   1. Use a standard mold with an effective bottom area of 3.0 cm × 2.5 cm, and reserve a blank boundary area of 1.5 mm on each side.
   2. Use a 0.5 mm fine-point waterproof marker to draw nine equally spaced parallel lines horizontally and vertically, forming a 9 × 9 positioning grid (81 intersection points in total).
      NOTE: Before starting, clean the mold interior with a xylene-soaked cotton swab to remove residual paraffin, which may cause delamination. During the drawing process, use a steel ruler to ensure uniform line thickness and clearly distinguishable intersection points.
2. Use a pipette to draw 5 µL of glue and gently place it at the center of the pre-labeled slide to form a single droplet. Immediately use fine tweezers to vertically pick up the tissue core at a 90° angle and slowly press it vertically into the glue drop (Figure 1C).
3. Insert the cores into the corresponding grid intersections on the mold using tweezers. Apply gentle pressure for 5 s to ensure secure adhesion (Figure 1D).
4. If the tissue core is displaced, fine-tune and reset it immediately using fine tweezers before the glue solidifies (within 30 s). Discard solidified, misaligned cores to maintain preparation quality while preserving precious samples to the greatest extent.

4. Cassette installation and paraffin embedding

1. Vertically place the tissue cassette over the steel mold, ensuring contact with the core tips without applying compression. Secure the cassette using magnetic clamps at all four corners, and seal the seams with molten paraffin.
2. Infuse molten paraffin at a 45° angle in a zigzag pattern, maintaining a flow rate of 1 mL/s. Ensure the paraffin level exceeds the core tips by 2 mm (Figure 1E).
3. Rapidly cool the block on a 4 °C cold plate for 5 min to form a rigid support layer. Transfer the block to a 22 °C environment for 20 min to reduce internal stress. Finally, use a 40 °C heat gun to gently smooth the surface and eliminate shrinkage marks.
4. Slightly heat the paraffin to soften it, then carefully cut along the cassette edges to loosen the block. Gently lift the cassette and remove any residual paraffin to ensure the integrity of the tissue cores (Figure 1F).

Results

In the present study, high-quality tissue microarrays were constructed using the glue method. To verify the effectiveness of the method, a series of experiments were performed, including H&E staining, immunohistochemical detection of specific proteins, and fluorescence in situ hybridization (FISH) analysis. A critical component of the construction process is the presence of tissue core dots at the expected positions and distances apart from one another, which is assessed by visual inspection. Visual inspecti...

Discussion

As an innovative method for constructing tissue microarrays (TMA), the glue method has shown significant advantages due to its ease of construction procedures and cost-effectiveness. Compared to the traditional needle array method, which relies on sophisticated instruments^14,15, the glue method enables sample fixation by glueing, which can be done with only basic tools, greatly reducing the technical threshold. In contrast to the traditional embedding method, the...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Breast cancer HER2 Detection kit	Anbiping	2502001	Breast cancer HER2 Detection kit
CDHR4 antibody	Abcam	ab166914	CDHR4 antibody
CDK1 antibody	Abcam	ab265590	CDK1 antibody
CRTAC1 antibody	Abcam	ab254691	CRTAC1 antibody
DNASE1L3 antibody	Abcam	ab203669	DNASE1L3 antibody
Embedding machine	P.S.J MEDICAL	BM450A	Embedding machine
Fully automatic tissue dehydrator	Leica Biosystems	ASP3005	Fully automatic tissue dehydrator
Glass microscope slides	Citotest	250124A1	Glass microscope slides
Glue	TIZO	200	Glue
GPR146 antibody	Abcam	ab117104	GPR146 antibody
IGSF10 antibody	Abcam	ab197671	IGSF10 antibody
ITIH1 antibody	Abcam	ab233032	ITIH1 antibody
Low Profile Microtome Blades	Thermo Fisher	3052835	Low Profile Microtome Blades
Marker pen	Deli	SK109	Marker pen
Microtome	Leica Biosystems	HistoCore BIOCUT	Microtome
Paraffin wax	Solarbio	YA0012	Paraffin wax
SMAD9 antibody	Abcam	ab262940	SMAD9 antibody
TARBP1 antibody	Abcam	ab115896	TARBP1 antibody
ZCCHC24 antibody	Abcam	ab88756	ZCCHC24 antibody