Green Synthesis of Magnesium Nanoparticles From Native Neotropical Savannah Plants For Scalable Nanophytocosmetic Formulations

Liryel Silva Gasparetto; Matheus Antônio Novaes; Cassiane Maria Vianna Campos; Juliana Alejandra Sanchez Torres; Vitor Hugo Brito Salentim; Lucas de Alcântara Sica de Toledo; Elizabete de Souza Cândido; Octávio Luiz Franco

doi:10.3791/70521

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Method Article

Green Synthesis of Magnesium Nanoparticles From Native Neotropical Savannah Plants For Scalable Nanophytocosmetic Formulations

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DOI:

10.3791/70521

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May 29th, 2026

Liryel Silva Gasparetto^*¹^,² , Matheus Antônio Novaes^*¹ , Cassiane Maria Vianna Campos¹ , Juliana Alejandra Sanchez Torres¹ , Vitor Hugo Brito Salentim¹ , Lucas de Alcântara Sica de Toledo¹ , Elizabete de Souza Cândido¹^,² , Octávio Luiz Franco¹^,²

¹S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, ²Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília

^* These authors contributed equally

Summary

This work describes a protocol for the green synthesis of magnesium nanoparticles using Stryphnodendron adstringens plant extract, a species from the Native Neotropical Savannah, followed by their incorporation and evaluation in a cosmetic serum formulation.

Abstract

This study describes a protocol for the green synthesis of magnesium-based nanoparticles using ethanol extracts of Stryphnodendron adstringens, a plant species native to the Neotropical savanna, and their incorporation into nanophytocosmetic formulations. The procedure includes collection and preparation of plant material, followed by ethanol extraction, in which phytochemicals act as reducing and stabilizing agents for magnesium ions. Nanoparticles are synthesized under controlled conditions using magnesium chloride and plant extracts, enabling an environmentally friendly process without the use of toxic reducing agents. Nanoparticle properties are characterized using dynamic light scattering and atomic force microscopy to assess size distribution, morphology, and colloidal behavior. The protocol further describes the incorporation of the synthesized nanoparticles into a cosmetic serum formulation. Stability is evaluated under controlled storage conditions by monitoring organoleptic properties, pH, and viscosity. This method provides a reproducible and sustainable approach for producing plant-mediated magnesium nanoparticles and assessing their application in cosmetic formulations.

Introduction

The Cerrado phytogeographic domain, which comprises the largest Neotropical savanna formation in South America, represents the most biodiverse tropical savanna region in the world. This ecosystem harbors thousands of plant species rich in secondary metabolites such as flavonoids, polyphenols, terpenes, and phenolic acids, many of which exhibit documented antioxidant, anti-inflammatory, and photoprotective activities of interest to the cosmetic and pharmaceutical industries¹^,²^,³^,⁴. These phytochemicals are widely incorporated as high-value ac....