Human brain organoids are three-dimensional brain-like tissue cultured from human embryonic stem cells or human induced pluripotent stem cells in vitro. Recent studies have shown that organoids recapitulate the various cell types found in vivo and the cytoarchitecture of a developing brain. This positions human brain organoids to be a promising candidate to model human neurodevelopment and neurodevelopmental diseases including autism. Organoids are an in vitro system, making them amenable to experimentation and accessible. This is very valuable especially in early developmental time-points where human tissue are scarce and obtaining samples at this age might present ethical issues. However, there have been concerns over reproducibility of the cultures as each laboratory performing these cultures would adapt protocols to best suits the cell lines they are working with. This is an important issue to resolve as there is already inherent variability that is present in using different cell lines for culture which might confound interpretation of data. The objective of this collection is to share standardized protocols for human brain organoid culture and its characterization. This, in turn will promote broader access to existing protocols and pave the way for research collaborations. We hope that this will encourage more scientists to consider using organoids as a model system aiding our efforts to further our understanding of neurodevelopment and neurodevelopmental diseases.