Cells are fundamental units of multi-cellular organisms. Inside each cell, small structures called organelles perform vital roles that support the function of cells, and in turn, the organisms. To understand their intricate functions, it is important to be able to visualize their dynamics or structures at high resolution. Organelles of interest include endosomes, mitochondria, endoplasmic reticulum, nucleus, Golgi, and lysosomes, to name a few. Also, viral particles are sometimes “unwanted” organelles that hijack cellular machinery. Visualization in intact organisms or cultured cells can be included in this collection. The aim of this collection is to include visualization protocols of the following interest: 1) how to label the organelles of interest? 2) How to track their dynamics in vivo or in cultured cells? 3) How to visualize them at super-resolution?
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1Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, 2Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 3Department of Pharmacy, Shanghai East Hospital, Tongji University
Methods for Visualizing Intracellular Organelles
Xiang Zhao*1
1University of California San Francisco, San Francisco, United States