Cnidarians are mostly marine invertebrates. During their life cycle, they switch between a sexually immature sessile polyp stage and a sexually matured swimming medusae stage. There is over a thousand cindarian species displaying many unique morphogenetic and behavioural features, which have made them fascinating systems to address a variety of developmental and evolutionary questions. To number just a few, their unique radial symmetrical body structures are ideal to study body plan evolution; both poylps and medusae forms have the ability to regenerate the lost tissues, which is complementary to vertebrate models to study whole-body regeneration. Cindarians have a simple nervous system architecture which eases the study of functions of neuronal cell types and evolution and the lack of senescence phenotype of polyps are unique to study aging processes.As non-standard model systems, the cellular-level studies of cnidarians heavily rely on the development and optimisation of protocols to culture and manipulate these animals. This collection aims to bring together the protocols that have been used for both the standardised methods to culture cnidarians and the newly developed cutting-edge technologies to visualise and functionally perturb developmental processes.