Renal blood flow (RBF) is a central parameter governing renal perfusion and function. RBF is finely regulated and autoregulated. In particular, it is regulated by multiple neurohormonal systems, such as the renin-angiotensin and prostaglandin systems. It also gets affected by heart failure., RBF is modulated by a large variety of drugs. Therefore, adequate and reproducible measurement of RBF is essential in rodent models of cardiovascular and renal diseases. For decades, researchers around the world have used perivascular probes to measure RBF in rodents. Depending on the experimental design, either acute measurements on anesthetized animals or measurements from probes chronically implanted in awake animals are obtained. Acute measurements are obtained relatively easily and allow for physiologic and pharmacologic manipulations, along with the simultaneous acquisition of other data. However, the confounding hemodynamic effects of various anesthetics are inevitable. Conversely, chronic RBF probe implantation and subsequent measurments on awake animals offer the precious advantage of removing the effect of anesthesia, while limiting experimental interventions. In this collection, standard methodologies are proposed to measure the RBF in rats and mice, acutely and chronically, in addition to RBF reserve and autoregulation.