Integral membrane proteins, which are encoded by 20-30% of all open reading frames and provide critical functions in the cell, account for around 60% of drug targets. However, only about 2-3% of their structures have been solved, putting them well behind their soluble counterparts. This is due to their hydrophobic nature, making it more challenging to express, isolate, and characterize. This collection protocol will provide the latest developments in membrane protein production, purification, characterization, and structure determination methods. This includes the modern protein expression techniques in various hosts, membrane protein solubilization screen, quality control for preparation of pure membrane proteins, and state-of-the-art approaches for studying structural and dynamic aspects of membrane proteins. The goal is to facilitate diverse techniques to develop a multifaceted approach to understand integral membrane proteins and their crucial biological processes.