Macroautophagy (autophagy) is a conserved process fundamental to all eukaryotic cells. It involves the isolation of cytoplasmic organelles and proteins into double-membrane vesicles and subsequent delivery of the cargo to the lysosome for degradation or recycling when there is a scarcity of nutrients. That makes autophagy a key catabolic process that is regulated by nutrient-sensing pathways and plays an important role in the regulation of the metabolic state of the cell. Autophagy, as well as metabolic changes, have been implicated in a variety of human pathologies, including neurodegenerative, metabolic, and gastrointestinal disorders, myopathies, and several cancer types, therefore a better understanding of the mechanisms through which autophagy functions to maintain metabolic homeostasis will not only provide a better insight into the pathogeny of those diseases but also into establishing novel therapeutic strategies. Drosophila melanogaster is a model organism that has been widely used in research to study and discover novel genes and signaling pathways. Its short lifespan and powerful genetic toolkit as well as the conservation of its genome across species, has made Drosophila ideal to study complex cellular processes at tissue and at an organismal level. This collection aims to highlight cellular, molecular, biochemical, and genetic approaches to study autophagy and metabolism in Drosophila animals and tissues at different developmental stages. The collection will provide detailed protocols of techniques that will be useful to scientists that are interested in studying autophagy and metabolism in Drosophila.