Ultrasound imaging offers a non-invasive and clinically feasible means to assess musculoskeletal structures in real-time. Recent research has implemented ultrasound imaging to assess structural and functional changes in anatomical structures as they relate to prevalent musculoskeletal injuries. Such studies have begun to use novel approaches to imaging, such as incorporating imaging during dynamic tasks and across functional movements of the sport. Given that many medical professionals have increasingly gained access to these technologies in their respective clinical sites, establishing consistent imaging methods is essential to advance assessment approaches. Furthermore, ultrasound imaging may be used in the context of real-time feedback to promote the recruitment and activation of musculature following a sports injury. This methods collection aims to synthesize recent advancements in musculoskeletal ultrasound imaging methods in the context of sports injury assessment and intervention. This information will provide an essential reference to healthcare professionals incorporating musculoskeletal ultrasound imaging into their clinical toolbox.