There is an emerging interest in utilizing adipose tissue-derived stem cells (ASCs) for regenerative therapies and procedures. ASCs are generally not administered alone but as a constituent of the stromal vascular fraction (SVF) in clinical practice. Numerous methods employing collagenase are available in the literature. While this approach yields an SVF containing many viable nucleated cells per gram of lipoaspirate, it is expensive, time-consuming, and tightly regulated due to safety concerns. As an alternative, mechanical dissociation methods have been developed to circumvent the barriers and challenges associated with the enzymatic approach, generating a minimally manipulated fat product. Given the importance of fat tissue for plastic surgery purposes, innovative mechanical methods could change how the surgeon conducts surgeries and improve surgical outcomes. In addition to the methods themselves, other research areas related to this topic include technologies and innovations related to fat collection (i.e., liposuction) and tissue scaffold engineering. This collection will be of particular interest to medical professionals interested in incorporating ASCs into their surgical procedures giving it great clinical relevance. Moreover, the video format offered by the Journal of Visualized Experiments is the perfect medium for demonstrating and describing these methods since mechanically based methods typically rely on shaking, vibration, centrifugation and/or washing, thus, facilitating the dissemination of this knowledge. Hoping that continued efforts in the research and development of mechanical adipose tissue dissociation methods will eventually bring in vivo human plastic surgery applications and regenerative medicine into the operating room.