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Visualization and Quantification of Leaf Anatomical Traits in C3 and C4 Plants

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Kumari Billakurthi

Kumari Billakurthi

University of Cambridge, Department of Plant Sciences

C4 plants possess specialized leaf anatomy, termed ‘Kranz’ anatomy. Enlarged bundle-sheath (BS) cells with numerous chloroplasts are two critical traits associated with the evolution of C4 leaf anatomy. Dr. Billakurthi’s present research is focused on dissecting the genetic regulators that specify C3 vs C4 BS anatomy using the monocot model plant Oryza sativa (Rice). Before joining the University of Cambridge, she completed her PhD at Heinrich Heine University, Germany. Her PhD thesis was about finding gene regulatory networks that determine C4 BS anatomy by forward genetic and reverse genetic approaches. 

Chiara Perico

Chiara Perico

University of Oxford, Department of Biology

Dr. Perico obtained her PhD from the University of Bristol (UK), working on the cytoskeletal factors driving plant organelle movement and positioning in Arabidopsis and tobacco. To unravel such mechanisms, she used a combination of cell biology, biochemistry and confocal microscopy techniques. She currently works as a post-doc in Prof Jane Langdale’s lab at the University of Oxford, where she is applying her cell biology and microscopy expertise to underpin the role of hormones in vein development. In particular, her research focuses on understanding the role of auxin on the venation patterning in leaves of C3 and C4 grasses, using Oryza sativa as a model C3 plant and Zea mays as representative of C4 species.

Sovanna Tan

Sovanna Tan

University of Oxford, Department of Biology

Unlike the diverse reticulated vein networks that develop in the variably shaped leaves of eudicotyledonous plant species, monocotyledonous grass leaves are unified by a linear shape and a pattern of parallel longitudinal veins. However, two variants of the venation pattern exist the derived ‘C4’ pattern, in which pairs of veins can be separated across the mediolateral leaf axis by just four intervening cells, and the ancestral ‘C3’ pattern with more widely spaced veins. After a PhD and a 1-year of assistant lectureship working on the hormonal regulation of plant development at the University of Paris-Saclay (France), Dr. Tan joined the Jane Langdale lab to understand what mechanisms underpin this constrained pattern with a focus on the relationship between cell divisions and vein formation in developing grass leaves. 

Collection Overview

C4 plants have evolved from the C3 ancestral state by acquiring changes in leaf anatomy and biochemistry. High vein density and enlarged bundle-sheath cells with numerous chloroplasts are the major anatomical traits associated with the specialized C4 leaf anatomy, termed ‘Kranz’ anatomy. In C4 Kranz species, each bundle-sheath cell is in direct contact with the mesophyll cell. This organization has facilitated the distribution of carbon assimilation reactions between mesophyll and bundle-sheath cells. To maintain the metabolite flux between these two cell types, the plasmodesmata number increased at the mesophyll-bundle-sheath cell interface. Establishing the C4 Kranz anatomy is a prerequisite to achieving the ambitious goal of engineering C4 photosynthesis into C3 crops. However, our understanding of Kranz anatomy regulation is still rudimentary. This methods collection focuses on simple and robust methods to visualize and quantify mesophyll and bundle-sheath cell dimensions, chloroplasts, veins and plasmodesmata connections in C3 and C4 species, which would be helpful for comparative studies of C3 and C4 plants. 

Articles

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
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Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

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Cited by 1

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2023

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Abstracts

Leaf plasmodesmata quantification using three-dimensional imaging

Florence Danila*1

1The Australian National University

Leaf hacks: Practical methods to studying young rolled leaves in grasses

Janlo Robil*1,

Paula McSteen1

1Quezon City, Philippines

Exploring C4 leaf cross sections viewed both perpendicular and parallel to the midvein

Moonsub Lee1,

Ryan Boyd1,

Donald Ort*1

1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana Champaign

Quantifying Light-Dependent Anatomical Plasticity in C₄ Maize Leaves

Tiina Tosens*1

1prof