The microbial community is ubiquitous in all ecosystems on earth, playing a crucial role in many processes of extreme importance to humankind. Over the last few decades, there has been an increased interest in studying microorganisms and their roles in various fields of microbiology, including environmental, medical, food, and industrial microbiology. 

Fluorescence in-situ hybridization (FISH) is a versatile molecular biology technique that allows the detection, quantification, and localization of specific DNA and RNA sequences within microbial cells, making it a powerful tool for microbial studies. However, FISH techniques can also present certain challenges, such as in sample preparation, probe design, optimization of hybridization conditions, and image analysis, which can impact the accuracy and reliability of results. 

This Methods Collection aims to provide a comprehensive overview of the current FISH protocols, including applications, probes, and modifications, and their relevance in different fields of microbiology.

Topics of interest include but are not limited to the following:

• FISH applications in environmental microbiology
• FISH applications in medical microbiology
• FISH applications in food microbiology
• FISH applications in industrial microbiology
• FISH probes and modifications
• Challenges and troubleshooting in FISH techniques

Overall, this collection will be a go-to resource for researchers in different fields of microbiology to select the best FISH protocol for their specific research needs and troubleshoot any issues that may arise during experimentation, leading to more efficient and targeted microbiological research.