Guest Editors
Katelyn Arnold
The University of North Carolina Chapel Hill, Eshelman School of Pharmacy, Division of Chemical Biology and Medicinal Chemistry
Dr. Katelyn Arnold is an assistant professor at the University of North Carolina Chapel Hill focused on biological applications of glycans generated from chemoenzymatic synthesis. Past work during my doctoral dissertation and the post-doctoral position includes the investigation of synthetic heparin/heparan sulfate oligosaccharides in acute inflammatory and thromboinflammatory disease models. Dr. Arnold’s long-term research goals include developing synthetic oligosaccharide therapeutics for acute and critical care diseases. Recently, Dr. Arnold was recognized by Forbes Magazine on the 2023 “30 Under 30” Science list for her work in glycoscience.
Mahesh Neralkar
Brandeis University, Waltham, MA, USA
Dr. Mahesh Neralkar has over 8 years of experience in chemical glycobiology. He completed his doctoral studies with Prof. Srinivas Hotha at IISER, Pune in India in 2019, and is currently a Postdoctoral Fellow working in the lab of Prof. Isaac Krauss at Brandeis University, Waltham, MA, USA. His dissertation explored the development of glycosyl donors and higher oligosaccharides synthesis from the cell wall of Mycobacterium tuberculosis. Currently, he is working on mRNA display evolved glycopeptide-based HIV-1 vaccine design. He has demonstrated large-scale synthesis of Man9GlcNAc2 glycan and connecting to peptide backbone using copper-assisted alkyne azide click chemistry to form glycopeptides. He uses techniques such as biolayer interferometry (BLI) and Saturated transfer difference (STD) NMR for the validation of synthetic glycopeptides as antigens for HIV-1 vaccines.
Collection Overview
Glycans are composed of monosaccharides linked together by glycosidic bonds to form oligosaccharides or polysaccharides. Glycans play essential roles in both health and disease states. The broad functionality of endogenous glycans arises from their structural and locational diversity. Structural diversity arises from different monosaccharide building blocks, α or β glycosidic linkages, and biosynthetic enzymes responsible for modifications. Locational diversity refers to the various glycan locations including in the extracellular matrix, on cell surfaces, in the cytoplasm and nucleus and conjugated to proteins and lipids. The field of glycobiology integrates carbohydrate chemistry and molecular biology.
This Methods Collection aims to include methods of endogenous glycan detection, structural analysis and function, and using glycan-based tools to study glycan-binding protein interactions.
Articles
Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
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Cited by 3
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2024
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