The study of cell death has spanned many years of scientific inquiry. Cell death is a vital biological process that helps maintain balance in cellular organisms. It plays a role in tissue renewal, immune response shaping, and embryonic development. Recent research has revealed new cell death pathways, notably ferroptosis—a unique form of iron-mediated cell death. Unlike other forms of cell death, ferroptosis operates independently of known regulatory pathways and is closely tied to cellular metabolism and redox balance. This discovery has filled gaps in our understanding of mammalian metabolism and has implications for drug discovery in areas like cancer, neurodegenerative diseases, and infectious diseases.

This Methods Collection aims to introduce scientists to ferroptosis and offer methods for its detection and analysis, spanning from metabolic analysis, phenotype identification and quantification, and assessing ferroptosis biomarkers in cells or tissues. By presenting a range of techniques, we equip researchers to explore ferroptosis in various experimental contexts. We aim to enhance our understanding of this novel cell death pathway and catalyze innovative work in this scientific field.