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Current Methods for Investigating Microbiota Interactions in Systemic Disorders
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Samar Rezq

Samar Rezq

University of Mississippi Medical Center, Department of Pharmacology

Dr. Samar Rezq is an assistant professor in the Department of Pharmacology at the University of Mississippi Medical Center (UMMC). She got her B.Sc. in Pharmaceutical Sciences in 2005 and M.Sc. in Pharmaceutical Sciences in 2010 from the Faculty of Pharmacy, Zagazig University, Egypt. She earned her PhD in Pharmacology and Toxicology from Brody School of Medicine, East Carolina University, NC, USA, in 2016. Her PhD project involved investigating the potential roles of central GPR109A and the prostanoid EP3 receptor in cardiovascular regulation using in vivo and in vitro models. Her postdoctoral research at UMMC focused on the part of microRNA-21 in metabolic derangements in Polycystic Ovary Syndrome (PCOS), the most common endocrine disorder in reproductive-age women; the role of obesity in microbiome, energy balance, and bile acid signaling in PCOS; and the role of microRNA-21 in energy balance. Dr. Rezq's current research elucidates the specific signaling pathways involved in PCOS-associated renal injury. 

Eman Soliman

Eman Soliman

Virginia Polytechnic Institute and State University, Department of Biomedical Sciences and Pathobiology

Dr. Eman Soliman is a research scientist in the Department of Biomedical Sciences and Pathobiology at Virginia Polytechnic Institute and State University (Virginia Tech). Her primary research interest is modulating the innate immune response to mitigate detrimental effects following traumatic brain injury (TBI). Her investigations are centered on elucidating the role of efferocytosis, the clearance of apoptotic debris, as a neuroprotective signal during the acute phase after brain trauma, before the initiation of maladaptive immune responses. 

Collection Overview

The microbiota plays a pivotal role in health and disease. Depending on localized regions, microbiota can be classified into gut, oral, respiratory, and skin microbiota, which actively contribute to homeostasis and orchestrate immune responses. However, dysbiosis of the microbiota results in the dysregulation of organ functions and the development of diseases such as cardiovascular diseases (CVDs), kidney diseases, respiratory diseases, neuropsychiatric diseases, and cancer.

The microbiome's plasticity, achieved by altering composition, function, or both, makes it a novel therapeutic target for different disorders. This comprehensive Methods Collection provides a dynamic exploration of recent approaches for studying microbiota and their intricate interactions with biological systems, advancing our understanding of the critical role of microbiota in various physiological and pathological contexts.

A broad spectrum of contributions is welcome, including but not limited to:

·      In Vivo Test and Imaging Procedures: innovative methods for testing within live organisms and advanced imaging techniques to reveal dynamic interactions.

·      Cell and Tissue Isolation Protocols: strategies for extracting microbiota, cells, and tissues with precision and reliability.

·      Sequencing Approaches: methodologies to analyze microbiota composition and functional implications.

·      Cellular Ex Vivo and In Vivo Analyses: exploring responses and reactions in controlled ex vivo settings and complex living organisms, unraveling microbiota-driven mechanisms.

·      Microbiota in Disease Treatment: illustrating the application of microbiota for disease management, utilizing techniques like microbiota modulation and fecal microbial transplantation.

Articles

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
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Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

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2025

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Abstracts

Salivary Microbiome Collection and Sequencing Protocol at Qatar Biobank

Haya Abuhijleh1,

Zain Zakria1,

Ayat Hammad1,

Fatima Qafoud 2,

Sara AlResheq2,

Eleni Fthenou2,

Eiman Alkhayat3,

Linda Hannigan2,

Maha AlAsmakh*1

1Qatar University,

2Qatar BioBank,

3Qatar Biobank

Establishing Gut Microbiome Collection and Preservation Protocols at Qatar Biobank for Versatile Downstream Applications

Sara Alresheq1,

Fatima Qafoud*1,

Linda Hannigan1,

Eiman Alkhayat1,

Eleni Fthenou1,

Maha AlAsmakh2,

Haya Abuhijleh2,

Zain Zakria2,

Ayat Hammad2

1QPHI- Qatar Biobank,

2Qatar University