Insects, one of Earth's most abundant and diverse animal groups, inhabit nearly every ecosystem. Their robust innate immunity makes them valuable models for studying this aspect of immunity. 

As agricultural pests, they cause significant economic losses by damaging and destroying crops, which necessitates the development of innovative pest management techniques to control their proliferation. Insects also pose a substantial threat to human and animal health by acting as vectors for various pathogens, including viruses, bacteria, protozoa, and nematodes. Over 500 arthropod-borne viruses (arboviruses) have been identified, with more than 100 of them being harmful to humans. Consequently, insects play a pivotal role in researching and controlling these arboviruses. 

However, insects can also be of benefit to humans. For instance, there are over 2,100 edible insect species that humans consume, providing a rich source of protein. Insects and their derivatives contain natural compounds with diverse biological significance, including antibacterial, antifungal, antiviral, anticancer, antioxidant, anti-inflammatory, and immunomodulatory properties. Thus, insects hold promise as an alternative to conventional pharmaceuticals for disease treatment. Due to their short life cycles and genetic similarities to humans, insects are employed in medical research to investigate gene functions. 

This Methods Collection aims to provide a comprehensive range of protocols related to insect research across various domains. It encompasses fundamental insect research, agricultural pest management, arboviruses, insects in medical research, and various other aspects of entomology.