
Ulrike Lange
University Medical Center Hamburg-Eppendorf, Heinrich Pette Institute, Leibniz Institute for Experimental Virology
Ulrike Lange is a clinician in the Department of Anesthesiology at the University Medical Center Hamburg-Eppendorf and an independent postdoctoral researcher at the Heinrich Pette Institute, Leibniz Institute for Experimental Virology in Hamburg (Germany). After her PhD in the laboratory of Azim Surani at the University of Cambridge (UK), she completed her medical degree at the University of Freiburg (Germany). She has since followed a dual clinical and research career, qualifying as specialized anesthesiologist as well as developing a profile as independent researcher. Her research focuses on molecular phenomena in chronic HIV-1 infection, combining her expertise in chromatin biology and genome regulation with the field of molecular virology. One of the major objectives of her young research group is to understand the crosstalk between HIV-1-derived DNA and the human genome of infected target cells. Ultimately, she would like to improve our understanding of the pathomechanisms in chronic HIV infection and thereby contribute to the development of new strategies towards achieving remission in chronically HIV-infected patients.

Roxane Verdikt
University of California, Los Angeles
Roxane Verdikt is a Belgian molecular biologist who earned her PhD in 2019 at the Université Libre de Bruxelles in the service of molecular virology led by Pr. Carine Van Lint. Her thesis focused on the epigenetic and transcriptional regulation of Human Immunodeficiency Virus type 1 (HIV-1). Notably, she studied how DNA methylation accumulates on the HIV-1 promoter during latent infections. She also investigated enhancer-mediated control of gene expression by studying how HIV-1 genes are specifically expressed in macrophages. At the beginning of 2020, Roxane joined the laboratory of Pr. Patrick Allard at the University of California, Los Angeles to study epigenetic regulation in germ cell specification. Her main scientific interests reside in the regulation of gene expression in the context of host-pathogen interplay as well as in the context of adaptation to environmental cues.

Martin V. Hamann
Heinrich Pette Institute for Experimental Virology
Martin V. Hamann is a biochemist by education and obtained his PhD in Molecular Retrovirology at the Dresden University of Technology, Germany, in 2015. He developed vector systems for the transient delivery of mRNA molecules by retroviral particles with potential therapeutic application. Following his thesis, he joined several other laboratories to widen his scientific view but never lost his contact to viruses and his research focus of host-pathogen interactions. Since 2019, he is a Postdoctoral Fellow in laboratory of Professor Joachim Hauber at the Heinrich Pette Institute for Experimental Virology in Germany. His projects aim is to understand HIV-1 latency on an epigenetic scale as well as HIV-1 crosstalk with human endogenous retroviruses.
Viruses have evolved to infect a broad range of hosts, with significant impacts on the health and economy of human societies. In this context, the fundamental interrogation of viral-host interplay is of paramount importance for the development of therapeutic interventions. One interface in this relationship occurs at the epigenetic level and involves regulatory mechanisms at different layers of the chromatin architecture. These include epigenetic modulation of chromatin components, such as DNA and histone proteins, as well as chromatin-associated protein scaffolds and non-coding RNAs. In addition, chromosomal and nuclear architecture, further components of epigenetic regulation, also appear involved in viral-host interplay upon infection.
In this special collection, we bring forward different techniques to investigate the impact of viral infections on host chromatin at the epigenetic level as well as techniques elucidating epigenetic regulation of viral genomes. This methods collection aims to provide techniques that will address a broad range of epigenetic mechanisms in the context of viral infections.
0 Views
•
Cited by 9
•
2020
•
Anna Kula-Pacurar1, Jakub Wadas2, Agnieszka Suder1, Artur Szczepanski1,2, Aleksandra Milewska1,2, Marek Ochman3,4, Tomasz Stacel3,4, Krzysztof Pyrc1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases
Exploring m6A and m5C epitranscriptomes upon viral infection: an example with HIV
Angela Ciuffi1,
Paolo Angelino2,
Sara Cristinelli*1
1Institute of Microbiology, Lausanne University Hospital and University of Lausanne, CH-1011 Lausanne, Switzerland,
2BCF - Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, CH-1015 Switzerland
Chromatin extraction from Frozen Tissue for ChIP analysis from host and viral genome
Maura Dandri*1,
Andrea Pirosu*1,
Lena Allweiss1
1I. Department of Internal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Analysis of nucleosome positioning on HIV DNA using Capture-MNase-Seq
Motoki Takaku1,
Monsef Benkirane*2,
Shinichi Machida*2
1School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202-9061, United States.,
2Institut de Génétique Humaine. Université de Montpellier. Laboratoire de Virologie Moléculaire CNRS-UMR9002, 34000 Montpellier, France.