Nervous system tissues are delicate and fragile to traumatic injuries and non-traumatic injuries. Injuries to the nervous system tissues are significant causes of morbidity and mortality and lead to severe social and economic burdens. The central nervous and peripheral nervous systems are largely incapable of repairments after nerve injuries and this can induce permanent motor, sensory, or autonomic nerve dysfunctions. Therefore, it is of great importance to explore strategies to treat central and peripheral nerve injury.

Establishing visual and standardized laboratory techniques on nerve injury and regeneration will contribute to the clinical treatment of nerve injuries. The objective of the current collection is to introduce commonly used experimental methods to establish nerve injury models, conduct nerve suture or nerve bridge, and evaluate functional recovery of injured nerves. Considering that cells in the nervous cells, especially neurons and glial cells, play essential roles in nerve regeneration, experimental techniques of the isolation, purification, and functional examination of neurons and glial cells also fit the collection.