Fluorescence photonic microscopy is a powerful technology that allows the visualization and analysis of structure and dynamic processes. In recent years, photonic microscopy fluorescence techniques have been developed in many fields. It offers innovative equipment, allowing the observation of single molecules up to the scale of an organism. Life Sciences have seen many techniques developed to gain information and more resolution about 2D, 3D, live, or fixed samples. These techniques have allowed us to answer a large biological question in different samples. This Methods Collection will bring together a range of in vivo, ex-vivo imaging fluorescence techniques, development in optical microscopy, sample preparation techniques to increase resolution (e.g., Clearing, ExM), and image analysis in the field of life sciences.