Phytochemicals, which are plant secondary metabolites widely present in the human diet and medicinal botanicals, have served as the lead chemicals for new drug development and/or marketed as dietary supplements for decades.

Despite significant advancements in this field, the methodologies for extracting and analyzing phytochemicals remain challenging. Current techniques range from conventional solvent extraction to advanced methods such as supercritical fluid extraction. Chemical analysis often employs nuclear magnetic resonance spectroscopy or chromatography coupled with mass spectrometry. However, challenges persist, particularly those related to the instability of many phytochemicals, the influence of the matrix effect, and the need for method standardization and translation.

The overall goal of this Methods Collection is to provide a comprehensive toolkit, from in vitro to in vivo studies, for researchers to trace the journey of phytochemicals from plant to pill to patient. Specifically:

• Consolidate standardized, reproducible and validated methods for the extraction of phytochemicals from plant sources and biological samples, ensuring the integrity of the compounds of interest
• Collate analytical protocols for the accurate identification and quantification of phytochemicals (precursor compounds) in plant materials and their metabolites in biological systems after oral intake
• Address the challenges of analyzing complex mixtures and the bioconversion of phytochemicals within living organisms

This Methods Collection will be a valuable resource for researchers in pharmacognosy, pharmacology, medicinal chemistry, chemical biology, nutrition, and food science. By providing robust extraction and analysis methods, it will aid in discovering novel bioactive compounds, standardizing botanical preparations and supplements, supporting evidence-based dietary recommendations, and developing cost-effective plant-based health products.