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TOPICAL COLLECTIONS

Novel Methods in Manipulating and Interrogating Synaptic Plasticity
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Guest Editors

Stuart Greenhill

Stuart Greenhill

Aston University, Institute of Health and Neurodevelopment

Dr Stuart Greenhill, PhD, is a Senior Lecturer in Neuroscience at Aston University, UK. A member of the University’s Institute of Health and Neurodevelopment, his research is focused on how synaptic plasticity drives normal network function in the brain and how this can go wrong in conditions such as epilepsy and schizophrenia. He uses a variety of techniques to approach his research, especially in vitro and in vivo electrophysiology, molecular biology, and computational methods. Alongside his fundamental research work, he has a strong interest in technology development and materials science research to advance electrophysiological experiments and open up new ways of understanding how our brains and the networks within them work.

Douglas Caruana

Douglas Caruana

Aston University, College of Health and Life Sciences

Dr. Douglas Caruana, PhD, is a Lecturer in Neuroscience at Aston University and a synaptic neurophysiologist specializing in drug- and disease-related changes in brain chemistry affecting synapses and memory. It is widely accepted that experience-dependent modifications in synaptic function underlie information storage in the brain and that modulatory neurotransmitter systems play a central role in regulating this neuroplasticity. A fundamental problem remains, though, in understanding how dysfunctions in neuromodulation linked to brain pathology (e.g., epilepsy or depression) or drug abuse alter synaptic mechanisms essential to cognition. Dr. Caruana uses electrophysiology, pharmacology, molecular biology, and behavior to determine how modulatory neurotransmitters and nootropic compounds modify synapses in the hippocampus and parahippocampal cortices to affect sensory and mnemonic processing in normal and diseased brains.

Collection Overview

Synaptic plasticity is one of the most critical and complex processes in biology. The basis of learning, memory, and skill acquisition is the ability of neuronal connections to re-map and change their strengths and weights individually, as well as for whole cells to adjust their synaptic strength multiplicatively. This process also underpins healthy neurodevelopment and can often be a key factor in developing aberrant neuronal activity in various conditions and disease states. One of neuroscience's fundamental techniques is to control, manipulate, and research the molecular, cellular, and functional components driving plasticity. 

This Methods Collection aims to highlight and disseminate recent developments in the field that can be used to manipulate or induce synaptic plasticity processes, record changes in cellular and network function, and analyze the observed changes. It includes detailed descriptions and video explanations on how to deploy these techniques to ensure maximum repeatability. We aim to illustrate methods for investigating plasticity both in vivo and in vitro and show how these approaches can drive translational medical research and enhance our understanding of healthy brain development.

Articles

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
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Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex

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Cited by 1

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2025

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Abstracts

Methods to study circadian plasticity of synapses

Anbarieh Saadat1,

Malgorzata Jasinska2,

Elzbieta Pyza*1

1Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University,

2Department of Histology, Jagiellonian University Medical College