New experimental models that recapitulate host-virus responses and pathological processes in humans are required for investigating viral disease and preparing for potential pandemics resulting from evolving viral infections. Organoids are promising in vitro tools that can overcome the limitations of standard 2D cell lines and animal models. These "mini-organs", derived from adult stem cells (ASCs) and human induced pluripotent stem cells (hiPSCs), offer significant advantages for studying organ development, disease modeling, and testing potential therapies. However, the absence of an interconnected vasculature network generally limits their translational potential and capacity to replicate complex in-vivo functions. 

This Methods Collection will focus on the following key topics: 

• Integrating organoids with different types of vascularized cells 
• Co-culturing lineage-specific organoids 
• Co-differentiating hiPSCs into vascular and organ-specific lineages 
• Incorporating perfusable vasculature within organoids using organoid-on-a-chip 
• 3D bioprinting for studying immune cell transmigration during infection 

Furthermore, the collection will highlight the insights that organoid models have contributed to viral research, discuss the limitations of current platforms, and explore emerging approaches for addressing these challenges.